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Product Info Summary
| SKU: | A02697 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Myosin-3 MYH3 Antibody
SKU/Catalog Number
A02697
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Myosin-3 MYH3 Antibody (Catalog # A02697). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Myosin-3 MYH3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02697)
Host
Rabbit
Contents
MYH3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MYH3 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH3. The immunogen is located within amino acids 30 - 80 of MYH3.
Cross-reactivity
MYH3 antibody is human, mouse and rat reactive. MYH3 antibody is predicted to not cross-react with other members of the myosin heavy chain family.
Reactive Species
A02697 is reactive to MYH3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
223.9 kDa
Background of MYH3
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH3 (myosin, heavy chain, skeletal muscle, embryonic) plays a significant role in skeletal muscle development (2) and is also essential for the proper morphology and function of the developing heart (3). Mutations in this gene have been associated with Freeman-Sheldon syndrome and Sheldon-Hall syndrome (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02697 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MYH3 antibody can be used for detection of MYH3 by Western blot at 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MYH3 in fetal human skeletal muscle lysate with MYH3 antibody at 1 μg/ml.
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Immunohistochemistry of MYH3 in mouse skeletal muscle tissue with MYH3 antibody at 5 μg/ml.
Specific Publications For Anti-Myosin-3 MYH3 Antibody (A02697)
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