Anti-MYPT1/PPP1R12A (Phospho-T696) Antibody (Monoclonal, 34P36)

Product Name

Anti-MYPT1/PPP1R12A (Phospho-T696) Antibody (Monoclonal, 34P36)

View all PPP1R12A Antibodies

SKU/Catalog Number

M01743

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-MYPT1/PPP1R12A (Phospho-T696) Antibody (Monoclonal, 34P36) catalog # M01743. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MYPT1/PPP1R12A (Phospho-T696) Antibody (Monoclonal, 34P36) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01743)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

34P36

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr696 of Human MYPT1.

Reactive Species

M01743 is reactive to PPP1R12A in Human, Mouse, Rat

Calculated molecular weight

115.3 kDa

Background of PPP1R12A

PPP1R12A (Protein phosphatase 1 regulatory subunit 12A), also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. PPP1R12A is one of the subunits of myosin phosphatase. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. The PPP1R12A gene is mapped on 12q21.2-q21.3. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in two ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01743 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information PPP1R12A

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