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- Table of Contents
NDUFB8 antibody (A07936-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of HEL cells using anti-NDUFB8 antibody (A07936-1).
Overlay histogram showing HEL cells stained with A07936-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB8 Antibody (A07936-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEL cells stained with A07936-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB8 Antibody (A07936-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-NDUFB8 Antibody Picoband®
NDUFB8 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
NDUFB8 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human A549 whole cell, human HepG2 whole cell, human Caco-2 whole cell, human U937 whole cell, human PC-3 whole cell, human HL-60 whole cell, rat liver tissue, rat heart tissue, rat lung tissue, mouse liver tissue, mouse heart tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NDUFB8 antibody was positively detected by IHC in:
human laryngeal squamous cell carcinoma tissue, human renal clear cell carcinoma tissue, human SM fatty carcinoma of the left kidney tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
NDUFB8 antibody was positively detected by ICC/IF in:
HepG2 cell
NDUFB8 antibody was positively detected by FC in:
HEL cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-NDUFB8 Antibody Picoband®
View all NDUFB8 Antibodies
View all NDUFB8 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NDUFB8 recombinant protein (Position: M1-I186).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
19-22 kDa
Calculated Molecular Weight
21.8 kDa
Background of NDUFB8
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial is an enzyme that in humans is encoded by the NDUFB8 gene. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cite This Product
Anti-NDUFB8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07936-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
93%
High Homology
Rabbit
85%
Moderate Homology
Cow
85%
Moderate Homology
Sheep
84%
Moderate Homology
Dog
84%
Moderate Homology
Rat
83%
Moderate Homology
Mouse
81%
Moderate Homology
Zebrafish
66%
Low homology
Drosophila
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NDUFB8 antibody
Immunohistochemistry (IHC) protocol for NDUFB8 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for NDUFB8 antibody
Flow Cytometry (FC) protocol for NDUFB8 antibody
Publications
Application:
Species:
Oocytes maintain low ROS levels to support the dormancy of primordial follicles
Published: 2024-09-19
Journal: AGING CELL
Application: IF
Impact Factor: 8.00
Species: Mouse
PMID: 39297300
Targeting oxidative phosphorylation to increase the efficacy of immune-combination therapy in renal cell carcinoma
Published: 2024-02-01
Journal: Journal for ImmunoTherapy of Cancer
Application: IHC
Impact Factor: 10.30
Species: Human
PMID: 38355278
Review
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Product Promise
We promise Anti-NDUFB8 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A07936-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026