Anti-Kir6.1/KCNJ8 Antibody Picoband™

Product Name

Anti-Kir6.1/KCNJ8 Antibody Picoband™

View all Kir6.1 Antibodies

SKU/Catalog Number

A04950-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Kir6.1/KCNJ8 Antibody Picoband™ catalog # A04950-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Kir6.1/KCNJ8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04950-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Kir6.1/KCNJ8 recombinant protein (Position: M1-S424).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04950-1 is reactive to KCNJ8 in Human, Mouse, Rat

Applications

A04950-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

48 kDa

Calculated molecular weight

47.968kDa

Background of Kir6.1

Potassium inwardly-rectifying channel, subfamily J, member 8, also known as KCNJ8, is a human gene encoding the Kir6.1 protein. Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. Defects in this gene may be a cause of J-wave syndromes and sudden infant death syndrome (SIDS).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Kir6.1/KCNJ8 using anti-Kir6.1/KCNJ8 antibody (A04950-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kir6.1/KCNJ8 antigen affinity purified polyclonal antibody (Catalog # A04950-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kir6.1/KCNJ8 at approximately 48KD. The expected band size for Kir6.1/KCNJ8 is at 48KD.

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Figure 2. IHC analysis of Kir6.1/KCNJ8 using anti-Kir6.1/KCNJ8 antibody (A04950-1).
Kir6.1/KCNJ8 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Kir6.1/KCNJ8 Antibody (A04950-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. Flow Cytometry analysis of U20S cells using anti-Kir6.1/KCNJ8 antibody (A04950-1).
Overlay histogram showing U20S cells stained with A04950-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Kir6.1/KCNJ8 Antibody (A04950-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For KCNJ8 (Source: Uniprot.org, NCBI)

Alternative Names

ATP-sensitive inward rectifier potassium channel 8; Inward rectifier K(+) channel Kir6.1; inwardly rectifying potassium channel KIR6.1; Kir6.1; Potassium channel, inwardly rectifying subfamily J member 8; potassium inwardly-rectifying channel, subfamily J, member 8; uKATP-1 KCNJ8 KIR6.1, uKATP-1 potassium inwardly rectifying channel subfamily J member 8 ATP-sensitive inward rectifier potassium channel 8|inward rectifier K(+) channel Kir6.1|inwardly rectifying potassium channel KIR6.1|potassium channel, inwardly rectifying subfamily J member 8|potassium voltage-gated channel subfamily J member 8

For more info on KCNJ8, check out the KCNJ8 Infographic

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In this infographic, you will see the following information for KCNJ8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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