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Product Info Summary
| SKU: | A06730 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NEGR1/Kilon Antibody
SKU/Catalog Number
A06730
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NEGR1/Kilon Antibody catalog # A06730. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEGR1/Kilon Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06730)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NEGR1. AA range:172-221
Reactive Species
A06730 is reactive to NEGR1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06730 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using NEGR1 Polyclonal Antibody
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Western blot analysis of lysates from COLO cells, using NEGR1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from COLO205 cells using NEGR1 antibody.
Specific Publications For Anti-NEGR1/Kilon Antibody (A06730)
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4 Customer Q&As for Anti-NEGR1/Kilon Antibody
Question
I was wanting to use your anti-NEGR1/Kilon antibody for WB for rat occipital lobe on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat occipital lobe identification?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
It shows on the product datasheet, A06730 anti-NEGR1/Kilon antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat occipital lobe in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-14
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for occipital lobe using anti-NEGR1/Kilon antibody A06730. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-17
Question
We are currently using anti-NEGR1/Kilon antibody A06730 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
The anti-NEGR1/Kilon antibody (A06730) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-15
Question
Would anti-NEGR1/Kilon antibody A06730 work for WB with occipital lobe?
Z. Williams
Verified customer
Asked: 2013-09-16
Answer
According to the expression profile of occipital lobe, NEGR1 is highly expressed in occipital lobe. So, it is likely that anti-NEGR1/Kilon antibody A06730 will work for WB with occipital lobe.
Boster Scientific Support
Answered: 2013-09-16
