Anti-Netrin 1/NTN1 Antibody
Rabbit IgG polyclonal antibody for Netrin-1(NTN1) detection. Tested with WB in Human;Mouse;Rat. Cited in 2 publication(s).
Anti-Netrin 1/NTN1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Netrin 1/NTN1 Antibody
See all Netrin-1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for NETRIN 1/NTN1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. NETRIN 1/NTN1 information: Molecular Weight: 67748 MW; Subcellular Localization: Secreted, extracellular space, extracellular matrix ; Tissue Specificity: Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level).|
|Cite This Product||Anti-Netrin 1/NTN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1662)|
|Similar Products From
|Anti-Netrin 1/NTN1 Antibody may replace the following items: sc 130773|sc 47889|sc 47888|sc 98876.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1(32-51aa AGQAAQPDPCSDENGHPRRC), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For NTN1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||9423|
|Species Of This Entry||Human|
|Alternative Names||Netrin-1|netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of|
|Post Tranlational Modifications||Phosphorylation|
|Subcellular Localization||Secreted. Cytoplasm. Mainly secreted.|
|Gene Location||17p13.1, on Chromasome 17, gene sequence: NC_000017.11 (9021510..9244000)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For NTN1 (Source: Uniprot.org, NCBI)
|Protein Function||Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.|
|Interacting Proteins||APP; DCC; DRAXIN|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Colorectal Cancer; Neoplasm Metastasis; Hepatitis B; Neoplasm Invasiveness; Adenocarcinoma; Liver Carcinoma; Hepatitis C; Prostatic Neoplasms; Inflammation; Nervousness; Tissue Adhesions; Malignant Neoplasm Of Breast; Ataxia; Hypoxia; Dysplasia; Tumor Angiogenesis; Acute-phase Reaction|
|Related Pathways||Cell Migration; Axon Guidance; Signal Transduction By P53 Class Mediator; Cell Adhesion; Negative Regulation Of Centriole Replication; Activation Of Mapk Activity; Cell Division; Cell Motility; Acute-phase Response; Cell Differentiation; Spinal Cord Development; Regulation Of Wnt Receptor Signaling Pathway; Cell Proliferation; Neutrophil Chemotaxis; Interleukin-8 Production; Humoral Immune Response; Regulation Of Peptidase Activity; Dna Replication; Cell Recognition; Regulation Of Centriole Replication|
|Background||Netrin-1(NTN1), also known as NTN1L, is a diffusible protein made by floor plate cells. Netrin-1 is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH. Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And netrin-1 expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually a netrin-1 receptor and induces cAMP accumulation on binding netrin-1, and that netrin-1-dependent outgrowth of dorsal spinal cord axons directly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a netrin-1 receptor or receptors function as conditional tumor suppressors. Expression of Unc5b triggered endothelial cell repulsion in response to netrin-1 in vitro, whereas a truncated Unc5b lacking the intracellular signaling domain failed to induce repulsion.|
|Scientific References||PMID: 9950216 by Meyerhardt J.A., et al. Netrin-1: interaction with deleted in colorectal cancer (DCC) and alterations in brain tumors and neuroblastomas.|
PMID: 20736409 by Li J., et al. Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins.
Our Boster Quality Guarantee for Anti-Netrin 1/NTN1 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: U87 Cell Lysate
Lane 2: COLO320 Cell Lysate
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Total number of citations: 2
|Qian-Hua W, Shao-Ping Z, Jian-Wen Z, Yun Y, Li Z. Mol Cell Biochem. 2011 Apr;350(1-2):81-7. Doi: 10.1007/S11010-010-0684-2. Epub 2010 Dec 31. Reduced Expression Of Netrin-1 Is Associated With Fetal Growth Restriction.||PubMed ID 21193949|
|Zhong S, Zou L, Zhao Y, Hu B, Xie H. J Huazhong Univ Sci Technolog Med Sci. 2010 Aug;30(4):500-4. Doi: 10.1007/S11596-010-0457-X. Epub 2010 Aug 17. Effect Of Different Concentrations Of Neogenin On Proliferation, Apoptosis And Related Proliferativ...||PubMed ID 20714878|
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