This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
NONO antibody (M03515) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Flow Cytometry analysis of U20S cells using anti-nmt55 p54nrb antibody (M03515).
Overlay histogram showing U20S cells stained with M03515 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-nmt55 p54nrb Antibody (M03515,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing U20S cells stained with M03515 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-nmt55 p54nrb Antibody (M03515,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Bundle Save
Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2)
NONO antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IF, ICC, WB |
Tested Applications
NONO antibody was positively detected by WB in:
human HELA whole cell, human placenta tissue, human MCF-7 whole cell, human A549 whole cell, human SW620 whole cell, human PANC-1 whole cell, human U20S whole cell, human K562 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NONO antibody was positively detected by ICC/IF in:
MCF-7 cell
NONO antibody was positively detected by ICC in:
A431 Cell
NONO antibody was positively detected by FC in:
U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunocytochemistry
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
5μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
Human
Tested
Monkey
Predicted
Rabbit
Predicted
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Rat
Predicted
Mouse
Predicted
Product Information
Product Name
Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2)
View all NONO Antibodies
View all NONO Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
11E2
Isotype
Mouse IgG1
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55 p54nrb, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
54.2 kDa
Background of NONO
Non-POU domain-containing octamer-binding protein is a protein that in humans is encoded by the NONO gene. This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
Cite This Product
Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03515)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
97%
High Homology
Cow
97%
High Homology
Sheep
97%
High Homology
Dog
97%
High Homology
Rat
94%
High Homology
Mouse
91%
High Homology
Zebrafish
40%
Low homology
Drosophila
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NONO antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for NONO antibody
Immunocytochemistry (ICC) protocol for NONO antibody
Flow Cytometry (FC) protocol for NONO antibody
Review
Have you used Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2)?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2) performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M03515
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026