Click image to see more details
Product Info Summary
| SKU: | PB9453 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Versican/VCAN Antibody Picoband®
SKU/Catalog Number
PB9453
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Versican/VCAN Antibody Picoband® catalog # PB9453. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Versican/VCAN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9453)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Versican, different from the related mouse and rat sequences by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9453 is reactive to VCAN in Human, Mouse, Rat
Observed Molecular Weight
373 kDa
Calculated molecular weight
372.8 kDa
Background of VCAN
Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9453 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: Rat Brain Tissue, HEPA Whole Cell
IHC: human glioma tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Vitronectin using anti-Vitronectin antibody (PB9453).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: HEPA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitronectin antigen affinity purified polyclonal antibody (Catalog # PB9453) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitronectin at approximately 373 kDa. The expected band size for Vitronectin is at 373 kDa.
Click image to see more details
IHC analysis of Vitronectin using anti-Vitronectin antibody (PB9453).
Vitronectin was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Vitronectin Antibody (PB9453) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Western blot analysis of versican/VCAN using anti-versican/VCAN Antibody (PB9453).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk with TBST for 1.5 hour at RT. The membrane was incubated with rabbit anti-versican/VCAN Antibody (PB9453) at 1: 500 overnight at 4℃ , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with an anti-rabbit 1 hour at room temperature. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for versican/VCAN at approximately 245 kDa. The expected band size for versican/VCAN is at 373 kDa.
Specific Publications For Anti-Versican/VCAN Antibody Picoband® (PB9453)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Versican/VCAN Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
1 Reviews For Anti-Versican/VCAN Antibody Picoband®
Working Antibody for Versican--Gang Ye, BIOLOGY, YORK UNIVERSITY, RESEARCH ASSOCIATE/LAB MANAGER
Excellent
Source: Biocompare.com
| SKU | PB9453 |
|---|---|
| Applications | Western Blot |
| Sample | Human cell lines |
| Detection | ECL |
"Since versican has several isoforms, the current antibodies targeting versican on the market is very tricky. Most of them didn't give us a reasonable result. Some of them only detect a band at 70 KD, which is unlikely a potent component of versican protein. Bosterbio claims this antibody dectects a band over 250 KD. Since versican is a very big protein. It sounds this is a real antibody. After testing, we found we can see a clear detected band around 245 KD. We realized that this might be the true result...Although 245 KD is still smaller than the versican core protein, it is likely the true result of versican dectection."
Gang Ye
Verified customer
Submitted 2016-08-24
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
18 Customer Q&As for Anti-Versican/VCAN Antibody Picoband®
Question
Do you provide bio tinylated version of PB9453?
Verified customer
Asked: 2021-11-03
Answer
Yes, we can provide bio tinylated version of the Anti-Versican/VCAN Antibody Picoband™ (PB9453).
Boster Scientific Support
Answered: 2021-11-03
Question
We have observed staining in mouse aortic smooth muscle. What should we do? Is anti-Versican/VCAN antibody supposed to stain aortic smooth muscle positively?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
Based on literature aortic smooth muscle does express VCAN. Based on Uniprot.org, VCAN is expressed in endometrium, placenta, glial tumor, brain, lung fibroblast, aortic smooth muscle, liver, among other tissues. Regarding which tissues have VCAN expression, here are a few articles citing expression in various tissues:
Aortic smooth muscle, Pubmed ID: 10397680
Brain, Pubmed ID: 7876137, 2466833, 2469524
Glial tumor, Pubmed ID: 7806529
Liver, Pubmed ID: 24275569
Lung fibroblast, Pubmed ID: 2820964
Placenta, Pubmed ID: 2583089
Boster Scientific Support
Answered: 2020-03-30
Question
We are currently using anti-Versican/VCAN antibody PB9453 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The anti-Versican/VCAN antibody (PB9453) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-03
Question
I see that the anti-Versican/VCAN antibody PB9453 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-04
Question
Will anti-Versican/VCAN antibody PB9453 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
According to the expression profile of brain, VCAN is highly expressed in brain. So, it is likely that anti-Versican/VCAN antibody PB9453 will work for WB with brain.
Boster Scientific Support
Answered: 2019-09-27
Question
My question regards to test anti-Versican/VCAN antibody PB9453 on rat brain for research purposes, then I may be interested in using anti-Versican/VCAN antibody PB9453 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
The products we sell, including anti-Versican/VCAN antibody PB9453, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-23
Question
Can you help my question with product PB9453, anti-Versican/VCAN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Yang
Verified customer
Asked: 2019-06-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9453 anti-Versican/VCAN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Versican/VCAN antibody PB9453. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-13
Question
Is this PB9453 anti-Versican/VCAN antibody reactive to the isotypes of VCAN?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
The immunogen of PB9453 anti-Versican/VCAN antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Versican (65-92aa KWSKIEVDKNGKDLKETTVLVAQNGNIK), different from the related mouse and rat sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-06
Question
Is a blocking peptide available for product anti-Versican/VCAN antibody (PB9453)?
Verified Customer
Verified customer
Asked: 2019-04-04
Answer
We do provide the blocking peptide for product anti-Versican/VCAN antibody (PB9453). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-04-04
Question
Is there a BSA free version of anti-Versican/VCAN antibody PB9453 available?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Versican/VCAN antibody PB9453 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-09
Question
I was wanting to use your anti-Versican/VCAN antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
As indicated on the product datasheet, PB9453 anti-Versican/VCAN antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-05
Question
My team were happy with the WB result of your anti-Versican/VCAN antibody. However we have seen positive staining in aortic smooth muscle extracellular space using this antibody. Is that expected? Could you tell me where is VCAN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-03-26
Answer
From literature, aortic smooth muscle does express VCAN. Generally VCAN expresses in secreted, extracellular space, extracellular. Regarding which tissues have VCAN expression, here are a few articles citing expression in various tissues:
Aortic smooth muscle, Pubmed ID: 10397680
Brain, Pubmed ID: 7876137, 2466833, 2469524
Glial tumor, Pubmed ID: 7806529
Liver, Pubmed ID: 24275569
Lung fibroblast, Pubmed ID: 2820964
Placenta, Pubmed ID: 2583089
Boster Scientific Support
Answered: 2018-03-26
Question
Here is the WB image, lot number and protocol we used for brain using anti-Versican/VCAN antibody PB9453. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-09
Question
Will anti-Versican/VCAN antibody PB9453 work on zebrafish IHC with glial tumor?
P. Li
Verified customer
Asked: 2015-11-27
Answer
Our lab technicians have not tested anti-Versican/VCAN antibody PB9453 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Versican/VCAN antibody PB9453 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish glial tumor in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-27
Question
We ordered your anti-Versican/VCAN antibody for IHC on brain a few months ago. I am using human, and We want to use the antibody for WB next. I was wanting to use examining brain as well as endometrium in our next experiment. Could give a recommendation on which antibody would work the best for WB?
J. Williams
Verified customer
Asked: 2014-11-27
Answer
I have checked the website and datasheets of our anti-Versican/VCAN antibody and it appears that PB9453 has been validated on human in both IHC and WB. Thus PB9453 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-11-27
Question
Does PB9453 anti-Versican/VCAN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Jackson
Verified customer
Asked: 2013-05-30
Answer
It shows on the product datasheet, PB9453 anti-Versican/VCAN antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-05-30
Question
Our lab want to know about using your anti-Versican/VCAN antibody for defective b4galt7 causes eds studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
S. Anderson
Verified customer
Asked: 2013-03-25
Answer
Thanks for your inquiry. This PB9453 anti-Versican/VCAN antibody is tested on rat brain tissue, tissue lysate, hepa whole cell lysate, human glioma tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-03-25
