Anti-NOSTRIN Antibody

Product Name

Anti-NOSTRIN Antibody

View all NOSTRIN Antibodies

SKU/Catalog Number

A06014

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NOSTRIN Antibody (Catalog # A06014). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

NOSTRIN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-NOSTRIN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06014)

Host

Rabbit

Contents

NOSTRIN antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NOSTRIN antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NOSTRIN. The immunogen is located within amino acids 430 - 480 of NOSTRIN.

Cross-reactivity

NOSTRIN antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all four isoforms.

Reactive Species

A06014 is reactive to NOSTRIN in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

57.7 kDa

Background of NOSTRIN

NOSTRIN. also known as endothelial nitric oxide synthase traffic inducer, is a member of the Pombe Cdc15 homology (PCH) family of proteins which may function as the modulator of the subcellular localisation and activity of endothelial nitric oxide synthase (eNOS) (1,2). It decreases NOS3 activity by inducing its translocation away from the plasma membrane (3). NOSTRIN typically exists as a trimer and is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, lung, kidney and heart. NOSTRIN is necessary for proper vascular development in zebrafish and postnatal retinal angiogenesis in mice (4,5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06014 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NOSTRIN antibody can be used for detection of NOSTRIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NOSTRIN

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