Anti-NADPH oxidase 1 NOX1 Antibody
Rabbit IgG polyclonal antibody for NADPH oxidase 1(NOX1) detection. Tested with WB, IHC-P in Human. Cited in 3 publication(s).
Anti-NADPH oxidase 1 NOX1 Antibody Info At A Glance
|Product Name||Anti-NADPH oxidase 1 NOX1 Antibody
See all NOX1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for NOX1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. NOX1 information: Molecular Weight: 64871 MW; Subcellular Localization: Cell projection, invadopodium membrane ; Multi-pass membrane protein ; Tissue Specificity: NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH- 1S is detected only in colon and colon carcinoma cells.|
|Cite This Product||Anti-NADPH oxidase 1 NOX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1739)|
|Similar Products From
|Anti-NADPH oxidase 1 NOX1 Antibody may replace the following items: sc 10183|sc 134389|sc 25545|sc 5819|sc 5821.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human NOX1(354-374aa, HIRAAGDWTENLIRAFEQQYS).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For NOX1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||27035|
|Species Of This Entry||Human|
|Protein Name||NADPH oxidase 1|
|Alternative Names||NOX1|EC 1.6.3; GP91-2; Mitogenic oxidase 1; MOX-1; MOX1NADH/NADPH mitogenic oxidase subunit P65-MOX; NADPH oxidase 1; NADPH oxidase homolog-1; NOH1; NOH1mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); NOH-1NADPH oxidase 1 variant NOH-1L; NOX1; NOX-1|
|Post Tranlational Modifications||Phosphorylation; Oxidation; Cleavage; Reduction; Dephosphorylation; Ubiquitination; Phosphorothioation; Nitration; Sumoylation; Carboxylation; Nitrosylation; Glycosylation; Deacetylation; Acetylation; Methylation|
|Subcellular Localization||Cell projection, invadopodium membrane; Multi-pass membrane protein. Cell membrane.|
|Gene Location||Xq22.1, on Chromasome X, gene sequence: NC_000023.11 (100843324..100874359, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For NOX1 (Source: Uniprot.org, NCBI)
|Protein Function||NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.|
|Tissue Specificity||NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH- 1S is detected only in colon and colon carcinoma cells.|
|Related Diseases||Hypertensive Disease; Inflammation; Malignant Neoplasms; Atherosclerosis; Neoplasms; Hypertrophy; Vascular Diseases; Cardiovascular Diseases; Fibrosis; Diabetes Mellitus; Tissue Adhesions; Tumor Angiogenesis; Carcinoma; Cell Transformation, Neoplastic; Hypoxia; Malignant Tumor Of Colon; Kidney Diseases; Colonic Neoplasms|
|Related Pathways||Cell Death; Pathogenesis; Cell Proliferation; Angiogenesis; Localization; Cell Migration; Cell Growth; Rna Interference; Transport; Secretion; Cell Cycle; Cell Adhesion; Electron Transport; Senescence; Inflammatory Response; Phagocytosis; Muscle Cell Proliferation; Smooth Muscle Cell Proliferation; Respiratory Burst; Gene Silencing|
|Background||NOX1(NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.|
|Scientific References||PMID: 10485709 by Suh Y.-A., et al. Cell transformation by the superoxide-generating oxidase Mox1.|
PMID: 10615049 by Banfi B., et al. A mammalian H+ channel, generated through alternative splicing of the NADPH oxidase homolog NOH-1.
Our Boster Quality Guarantee for Anti-NADPH oxidase 1 NOX1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
IHC(P): Human Lung Cancer Tissue
All lanes: Anti NOX1 (PA1739) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 65KD
Observed bind size: 65KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 3
|Wang Y, Wang Y, Liu D, Wang W, Zhao H, Wang M, Yin H. Carbohydr Polym. 2015 Jul 10;125:135-45. Doi: 10.1016/J.Carbpol.2015.02.012. Epub 2015 Feb 19. Cordyceps Sinensis Polysaccharide Inhibits Pdgf-Bb-Induced Inflammation And Ros Production In Huma...||PubMed ID 25857968|
|Gu Y, Gong Y, Zhang H, Dong X, Zhao T, Burczynski Fj, Wang G, Sun S, Zhu B, Han W, Wang H, Li P. Biochem Cell Biol. 2013 Jun;91(3):187-93. Doi: 10.1139/Bcb-2012-0087. Epub 2012 Dec 13. Regulation Of Transforming Growth Factor Beta 1 Gene Expressio...||PubMed ID 23668792|
|Deficiency of NOX1 or NOX4 Prevents Liver Inflammation and Fibrosis in Mice through Inhibition of Hepatic Stellate Cell Activation||PubMed ID 29963902|
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Note: sample size takes longer to process, average 5-7 days.
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