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Nox1 antibody (PA1666) performs well in IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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IHC analysis of NOX1 using anti-NOX1 antibody (PA1666).
NOX1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOX1 Antibody (PA1666) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NOX1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOX1 Antibody (PA1666) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-NADPH oxidase 1 NOX1 Antibody Picoband®
Nox1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
Nox1 antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Nox1 antibody was positively detected by IHC in:
rat colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-NADPH oxidase 1 NOX1 Antibody Picoband®
View all NOX1 Antibodies
View all NOX1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of rat NOX1, different from the related mouse sequence by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
65.2 kDa
Background of Nox1
NOX1 (NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.
Cite This Product
Anti-NADPH oxidase 1 NOX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1666)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
93%
High Homology
Rabbit
87%
Moderate Homology
Sheep
73%
Moderate Homology
Human
67%
Low homology
Cow
67%
Low homology
Monkey
67%
Low homology
Dog
53%
Low homology
Zebrafish
47%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Nox1 antibody
Immunohistochemistry (IHC) protocol for Nox1 antibody
Publications
Application:
Species:
Echinococcus granulosus-Induced Liver Damage Through Ferroptosis in Rat Model
Published: 2025-02-22
Journal: Cells
Application: WB
Impact Factor: 5.20
Species: Rat
PMID: 40072057
THRA1/PGC-1α/SIRT3 pathway regulates oxidative stress and is implicated in hypertension of maternal hypothyroid rat offspring
Published: 2024-12-06
Journal: HYPERTENSION RESEARCH
Application: WB
Impact Factor: 4.30
Species: Rat
PMID: 39639130
In utero chronic intermittent nicotine aerosol exposure increases ischemic heart injury in adult offspring via programming of Angiotensin II receptor-derived TGFβ/ROS/Akt signaling pathway
Published: 2024-06-28
Journal: REPRODUCTIVE TOXICOLOGY
Application: WB
Impact Factor: 3.30
Species: Rat
PMID: 38945500
Prenatal hypothyroidism diminished exogenous NO-mediated diastolic effects in fetal rat thoracic aorta smooth muscle via increased oxidative stress
Published: 2022-08-12
Journal: REPRODUCTIVE TOXICOLOGY
Application: WB
Impact Factor: 3.42
Species: Rat
PMID: 35970333
Cordycepin alleviated metabolic inflammation in Western diet-fed mice by targeting intestinal barrier integrity and intestinal flora
Published: 2022-03-25
Journal: PHARMACOLOGICAL RESEARCH
Application: WB
Impact Factor: 10.33
Species: Mouse
PMID: 35346845
Inhibition of neointima hyperplasia by the combined therapy of linagliptin and metformin via AMPK/Nox4 signaling in diabetic rats
Published: 2019-07-29
Journal: FREE RADICAL BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 5.66
Species: Rat
PMID: 31369842
Epigenetic Down-Regulation of Sirt 1 via DNA Methylation and Oxidative Stress Signaling Contributes to the Gestational Diabetes Mellitus-Induced Fetal Programming of Heart Ischemia-Sensitive Phenotype in Late Life
Published: 2019-05-11
Journal: International Journal of Biological Sciences
Application: WB
Impact Factor: 4.07
Species: Rat
PMID: 31223283
RAP-8 ameliorates liver fibrosis by modulating cell cycle and oxidative stress
Published: 2019-04-29
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 3.45
Species: Rat
PMID: 31047894
Role of NADPH oxidase pathway in renal protection induced by procyanidin B2: In L-NAME induced rat hypertension model
Published: 2018-06-15
Journal: Journal of Functional Foods
Application: IF
Impact Factor: 3.47
Species: Rat
Argirein alleviates vascular endothelial insulin resistance through suppressing the activation of Nox4-dependent O2- production in diabetic rats
Published: 2018-04-27
Journal: FREE RADICAL BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 6.02
Species: Rat
PMID: 29709706
Mechanisms of N‑acetylcysteine in reducing monocrotaline‑induced pulmonary hypertension in rats: Inhibiting the expression of Nox1 in pulmonary vascular smooth muscle cells
Published: 2017-08-22
Journal: Molecular Medicine Reports
Application: IF
Impact Factor: 1.69
Species: Rat
PMID: 28849167
The role of the Nox4-derived ROS-mediated RhoA/Rho kinase pathway in rat hypertension induced by chronic intermittent hypoxia
Published: 2017-01-11
Journal: Sleep and Breathing
Application: WB
Impact Factor: 2.29
Species: Rat
PMID: 28078487
Andrographolide ameliorates diabetic nephropathy by attenuating hyperglycemia-mediated renal oxidative stress and inflammation via Akt/NF-κB pathway
Published: 2016-07-01
Journal: MOLECULAR AND CELLULAR ENDOCRINOLOGY
Application: WB
Impact Factor: 3.86
Species: Mouse
PMID: 27378149
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Product Promise
We promise Anti-NADPH oxidase 1 NOX1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1666
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026