Product Info Summary
SKU: | PA1666 |
---|---|
Size: | 100μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-NADPH oxidase 1 NOX1 Antibody
SKU/Catalog Number
PA1666
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NADPH oxidase 1 NOX1 Antibody catalog # PA1666. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NADPH oxidase 1 NOX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1666)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of rat NOX1 (417-431aa WYKFQRAHNKLKTQK), different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross Reactivity
No cross reactivity with other proteins
Reactive Species
PA1666 is reactive to Nox1 in Mouse, Rat
Applications
PA1666 is guaranteed for IHC, WB Boster Guarantee
Background of Nox1
NOX1 (NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions

Click image to see more details
Anti-NOX1 antibody, PA1666, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Heart Tissue Lysate
Lane 4: Mouse Heart Tissue Lysate

Click image to see more details
Anti-NOX1 antibody, PA1666, IHC(P)
IHC(P): Rat Kidney Tissue
Protein Target Info & Infographic
Gene/Protein Information For Nox1 (Source: Uniprot.Org, NCBI)
Uniprot ID
Q9WV87
Gene ID
114243
Gene Name
Nox1
Full Name
NADPH oxidase 1
Weight
65.177kDa
Alternative Names
EC 1.6.3; GP91-2; Mitogenic oxidase 1; MOX-1; MOX1NADH/NADPH mitogenic oxidase subunit P65-MOX; NADPH oxidase 1; NADPH oxidase homolog-1; NOH1; NOH1mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); NOH-1NADPH oxidase 1 variant NOH-1L; NOX1; NOX-1 Nox1|NADPH oxidase 1|NADPH oxidase 1|MOX-1|MOX1|NADH/NADPH mitogenic oxidase subunit p65-mox|NOH-1|NOX-1|mitogenic oxidase 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Nox1, check out the Nox1 Infographic

We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Nox1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-NADPH oxidase 1 NOX1 Antibody (PA1666)
PA1666 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Deficiency of NOX1 or NOX4 Prevents Liver Inflammation and Fibrosis in Mice through Inhibition of Hepatic Stellate Cell Activation
Gu Y, Gong Y, Zhang H, Dong X, Zhao T, Burczynski Fj, Wang G, Sun S, Zhu B, Han W, Wang H, Li P. Biochem Cell Biol. 2013 Jun;91(3):187-93. Doi: 10.1139/Bcb-2012-0087. Epub 2012 Dec 13. Regulation Of Transforming Growth Factor Beta 1 Gene Expressio...
Wang Y, Wang Y, Liu D, Wang W, Zhao H, Wang M, Yin H. Carbohydr Polym. 2015 Jul 10;125:135-45. Doi: 10.1016/J.Carbpol.2015.02.012. Epub 2015 Feb 19. Cordyceps Sinensis Polysaccharide Inhibits Pdgf-Bb-Induced Inflammation And Ros Production In Huma...
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