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Product Info Summary
| SKU: | A03957 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NPT2b SLC34A2 Antibody
SKU/Catalog Number
A03957
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NPT2b SLC34A2 Antibody catalog # A03957. Tested in WB, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NPT2b SLC34A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03957)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from NPT2b . at AA range: 630-710
Reactive Species
A03957 is reactive to SLC34A2 in Human, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
75.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03957 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of extracts from rat kidney, K562 cells, using NPT2b Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-NPT2b SLC34A2 Antibody (A03957)
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Customer Q&As
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7 Customer Q&As for Anti-NPT2b SLC34A2 Antibody
Question
My question regarding product A03957, anti-NPT2b antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03957 anti-NPT2b antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-27
Question
Is there a BSA free version of anti-NPT2b antibody A03957 available?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NPT2b antibody A03957 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-13
Question
Would A03957 anti-NPT2b antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Johnson
Verified customer
Asked: 2019-06-26
Answer
You can see on the product datasheet, A03957 anti-NPT2b antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-26
Question
I see that the anti-NPT2b antibody A03957 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-28
Question
Does anti-NPT2b antibody A03957 work on monkey WB with intestine?
L. Zhang
Verified customer
Asked: 2018-01-09
Answer
Our lab technicians have not tested anti-NPT2b antibody A03957 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-NPT2b antibody A03957 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey intestine in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-09
Question
See below the WB image, lot number and protocol we used for small intestine using anti-NPT2b antibody A03957. Please let me know if you require anything else.
C. Johnson
Verified customer
Asked: 2015-12-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-15
Question
My lab would like to test anti-NPT2b antibody A03957 on human small intestine for research purposes, then I may be interested in using anti-NPT2b antibody A03957 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Carter
Verified customer
Asked: 2014-05-22
Answer
The products we sell, including anti-NPT2b antibody A03957, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-05-22
