Anti-NUR77/NR4A1 Antibody

NGFI-B alpha/Nur77/NR4A1 antibody

Boster Bio Anti-NUR77/NR4A1 Antibody catalog # PA1676. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1676
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-NUR77/NR4A1 Antibody

View all NGFI-B alpha/Nur77/NR4A1 Antibodies

SKU/Catalog Number

PA1676

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NUR77/NR4A1 Antibody catalog # PA1676. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NUR77/NR4A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1676)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human NUR77, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1676 is reactive to NR4A1 in Human, Mouse, Rat

Applications

PA1676 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

64.463kDa

Background of NGFI-B alpha/Nur77/NR4A1

NR4A1 (NUCLEAR RECEPTOR SUBFAMILY 4, GROUP A, MEMBER 1), also called NAK1, GFRP1, TR3, NUR77 or NGFIB, is a protein that in humans is encoded by the NR4A1 gene, which is also a member of the Nur nuclear receptor family of intracellular transcription factors. The NR4A1 gene is mapped on 12q13.13. NR4A1 is involved in cell cycle mediation, inflammation and apoptosis. It plays a key role in mediating inflammatory responses in macrophages. In addition, subcellular localization of the NR4A1 protein appears to play a key role in the survival and death of cells. Nr4a1 was overexpressed in Wnt1 -transformed mouse mammary cells. Nr4a1 was also induced by lithium, a Wnt1 mimic, and the Nr4a1 promoter was activated by lithium and beta-catenin, a Wnt1 downstream effector. In contrast, human NR4A1 was not upregulated by beta-catenin, indicating that this gene is regulated differently in human and mouse cells. Adenoviral expression of Nr4a1 induced genes involved in gluconeogenesis, stimulated glucose production both in vitro and in vivo, and raised blood glucose levels.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For NR4A1 (Source: Uniprot.org, NCBI)

Gene Name

NR4A1

Full Name

Nuclear receptor subfamily 4 group A member 1

Weight

64.463kDa

Superfamily

nuclear hormone receptor family

Alternative Names

Early response protein NAK1; GFRP1ST-59; growth factor-inducible nuclear protein N10; HMR; HMRNP10; hormone receptor; MGC9485; N10; NAK1; NAK-1; NGFIB alpha; NGFI-B alpha; NGFIB; NR4A1; Nuclear hormone receptor NUR/77; nuclear receptor subfamily 4 group A member 1; nuclear receptor subfamily 4, group A, member 1; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; steroid receptor TR3; Testicular receptor 3; TR3 orphan receptor; TR3 NR4A1 GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3 nuclear receptor subfamily 4 group A member 1 nuclear receptor subfamily 4 group A member 1|ST-59|TR3 orphan receptor|early response protein NAK1|growth factor-inducible nuclear protein N10|hormone receptor|nerve growth factor IB nuclear receptor variant 1|nuclear hormone receptor NUR/77|orphan nuclear receptor HMR|orphan nuclear receptor TR3|steroid receptor TR3|testicular receptor 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NR4A1, check out the NR4A1 Infographic

NR4A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NR4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-NUR77/NR4A1 Antibody

Question

I see that the anti-NUR77/NR4A1 antibody PA1676 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-02

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-02

Question

I was wanting to use your anti-NUR77/NR4A1 antibody for WB for human skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skeletal muscle identification?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

It shows on the product datasheet, PA1676 anti-NUR77/NR4A1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human skeletal muscle in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-13

Question

Will PA1676 anti-NUR77/NR4A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-04-08

Answer

You can see on the product datasheet, PA1676 anti-NUR77/NR4A1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-08

Question

Is this PA1676 anti-NUR77/NR4A1 antibody reactive to the isotypes of NR4A1?

Verified Customer

Verified customer

Asked: 2017-12-13

Answer

The immunogen of PA1676 anti-NUR77/NR4A1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human NUR77(99-112aa ASFKFEDFQVYGCY), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-13

Question

My question regarding product PA1676, anti-NUR77/NR4A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-12-05

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1676 anti-NUR77/NR4A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-12-05

Question

Does anti-NUR77/NR4A1 antibody PA1676 work on primate WB with fetal skeletal muscle?

Verified Customer

Verified customer

Asked: 2017-10-26

Answer

Our lab technicians have not validated anti-NUR77/NR4A1 antibody PA1676 on primate. You can run a BLAST between primate and the immunogen sequence of anti-NUR77/NR4A1 antibody PA1676 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate fetal skeletal muscle in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-26

Question

Do you have a BSA free version of anti-NUR77/NR4A1 antibody PA1676 available?

R. Zhao

Verified customer

Asked: 2015-08-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NUR77/NR4A1 antibody PA1676 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-08-17

Order DetailsPrice
PA1676

100μg

$370
PA1676-10ug

10μg sample (liquid)

$99
PA1676-Biotin

100 μg Biotin conjugated

$570
PA1676-Cy3

100 μg Cy3 conjugated

$570
PA1676-Dylight488

100 μg Dylight488 conjugated

$570
PA1676-Dylight550

100 μg Dylight550 conjugated

$570
PA1676-Dylight594

100 μg Dylight594 conjugated

$570
PA1676-FITC

100 μg FITC conjugated

$570
PA1676-HRP

100 μg HRP conjugated

$570
PA1676-APC

100 μg APC conjugated

$670
PA1676-PE

100 μg PE conjugated

$670
PA1676-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1676
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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