Anti-ORC5L/ORC5 Antibody Picoband™

Product Name

Anti-ORC5L/ORC5 Antibody Picoband™

View all ORC5L Antibodies

SKU/Catalog Number

A03676-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ORC5L/ORC5 Antibody Picoband™ catalog # A03676-1. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ORC5L/ORC5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03676-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ORC5, identical to the related mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03676-1 is reactive to ORC5 in Human, Monkey, Mouse, Rat

Applications

A03676-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

50.283kDa

Background of ORC5L

Origin recognition complex subunit 5 is a protein that in humans is encoded by the ORC5 (ORC5L) gene. The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ORC5L/ORC5 using anti-ORC5L/ORC5 antibody (A03676-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse ovary tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORC5L/ORC5 antigen affinity purified polyclonal antibody (Catalog # A03676-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORC5L/ORC5 at approximately 50 kDa. The expected band size for ORC5L/ORC5 is at 50 kDa.

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Figure 2. IF analysis of ORC5L/ORC5 using anti-ORC5L/ORC5 antibody (A03676-1).
ORC5L/ORC5 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ORC5L/ORC5 Antibody (A03676-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of U87 cells using anti-ORC5L/ORC5 antibody (A03676-1).
Overlay histogram showing U87 cells stained with A03676-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ORC5L/ORC5 Antibody (A03676-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ORC5 (Source: Uniprot.org, NCBI)

Alternative Names

ORC5Lorigin recognition complex, subunit 5-like (yeast); ORC5P; ORC5T; origin recognition complex subunit 5; origin recognition complex, subunit 5 (yeast homolog)-like; origin recognition complex, subunit 5 homolog (yeast); origin recognition complex, subunit 5; origin recognition complex, subunit 5-like ORC5 ORC5LP, ORC5T, PPP1R117, ORC5 origin recognition complex subunit 5 origin recognition complex subunit 5|protein phosphatase 1, regulatory subunit 117

For more info on ORC5, check out the ORC5 Infographic

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In this infographic, you will see the following information for ORC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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