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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-Ornithine Carbamoyltransferase/OTC Antibody Picoband™
Boster Bio Anti-Ornithine Carbamoyltransferase/OTC Antibody Picoband™ catalog # A00721-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ornithine Carbamoyltransferase/OTC Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00721-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human OTC (33-70aa NKVQLKGRDLLTLKNFTGEEIKYMLWLSADLKFRIKQK), different from the related mouse and rat sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00721-1 is reactive to OTC in Human, Mouse, Rat
A00721-1 is guaranteed for WB Boster Guarantee
Background of Ornithine Carbamoyltransferase
Ornithine transcarbamylase (OTC) (also called ornithine carbamoyltransferase) is an enzyme that catalyzes the reaction between carbamoyl phosphate (CP) and ornithine (Orn) to form citrulline (Cit) and phosphate (Pi). This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may also play a role in that disease.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of OTC using anti-OTC antibody (A00721-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTC antigen affinity purified polyclonal antibody (Catalog # A00721-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OTC at approximately 40KD. The expected band size for OTC is at 40KD.
Gene/Protein Information For OTC (Source: Uniprot.Org, NCBI)
Ornithine carbamoyltransferase, mitochondrial
aspartate/ornithine carbamoyltransferase superfamily
EC 2.1.3; EC 220.127.116.11; MGC129967; MGC129968; MGC138856; OCTD; Ornithine Carbamoyltransferase; ornithine carbamoyltransferase, mitochondrial; Ornithine Transcarbamylase; OTC; OTCase OTC OCTDD, OTC ornithine transcarbamylase ornithine transcarbamylase, mitochondrial|OTCase|ornithine carbamoyltransferase, mitochondrial*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on OTC, check out the OTC Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for OTC: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for A00721-1
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