Product Info Summary
SKU: | A00173T187 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-p27 Kip1 (phospho-T187) CDKN1B Antibody
SKU/Catalog Number
A00173T187
Size
100ul
Form
Liquid
Description
Boster Bio Anti-p27 Kip1 (phospho-T187) CDKN1B Antibody catalog # A00173T187. Tested in IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p27 Kip1 (phospho-T187) CDKN1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00173T187)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human p27 around the phosphorylation site of T157.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00173T187 is reactive to CDKN1B in Human, Mouse, Rat
Applications
A00173T187 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
22.073kDa
Background of p27/Kip1
Anti cdk2 antibody recognizes a protein encoded by a gene member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunohistochemistry validation of CDKN1B using Anti-p27 Kip1 (phospho-T187) CDKN1B Antibody (A00173T187).
Immunohistochemistry (IHC) analyzes of p-p27 Kip1 (T187) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibodyClick image to see more details
Figure 2. Immunohistochemistry validation of CDKN1B using Anti-p27 Kip1 (phospho-T187) CDKN1B Antibody (A00173T187).
Immunohistochemistry (IHC) analyzes of p-p27 Kip1 (T187) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibodyProtein Target Info & Infographic
Gene/Protein Information For CDKN1B (Source: Uniprot.org, NCBI)
Gene Name
CDKN1B
Full Name
Cyclin-dependent kinase inhibitor 1B
Weight
22.073kDa
Superfamily
CDI family
Alternative Names
CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1 CDKN1B CDKN4, KIP1, MEN1B, MEN4, P27KIP1 cyclin dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B|cyclin-dependent kinase inhibitor 1B (p27, Kip1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN1B, check out the CDKN1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p27 Kip1 (phospho-T187) CDKN1B Antibody (A00173T187)
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No publications found for A00173T187
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Customer Q&As
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1 Customer Q&As for Anti-p27 Kip1 (phospho-T187) CDKN1B Antibody
Question
Has A00173T187 been tested with IHC on prostate tissue?
Verified customer
Asked: 2020-12-23
Answer
Unfortunately, our lab hasn't tested the Anti-P27 Kip1 (Phospho-T187) CDKN1B Antibody (A00173T187) with IHC on prostate tissue.
Boster Scientific Support
Answered: 2020-12-23