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Product Info Summary
| SKU: | PA1102 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SKP2 Antibody Picoband®
SKU/Catalog Number
PA1102
BA2740 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SKP2 Antibody catalog # PA1102. Tested in Flow Cytometry, ICC/IF, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SKP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1102)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SKP2, different from the related rat and mouse sequences by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1102 is reactive to SKP2 in Human
Observed Molecular Weight
48 kDa
Calculated molecular weight
47.8 kDa
Background of SKP2
The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G (1)-S transition by controlling the stability of several G (1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1102 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human 293T whole cell, human Jurkat whole cell, human HepG2 whole cell, human MCF-7 whole cell
ICC/IF: SiHa cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of SKP2 using anti-SKP2 antibody (PA1102).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP2 antigen affinity purified polyclonal antibody (PA1102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SKP2 at approximately 48 kDa. The expected band size for SKP2 is at 48 kDa.
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IF analysis of SKP2 using anti-SKP2 antibody (PA1102) and anti-Tubulin Alpha antibody (M03989-3).
SKP2 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SKP2 Antibody (PA1102) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Flow Cytometry analysis of HepG2 cells using anti-SKP2 antibody (PA1102).
Overlay histogram showing HepG2 cells stained with PA1102 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SKP2 Antibody (PA1102, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-SKP2 Antibody Picoband® (PA1102)
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7 Customer Q&As for Anti-SKP2 Antibody Picoband®
Question
I see that the anti-SKP2 antibody PA1102 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oocyte using anti-SKP2 antibody PA1102. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-30
Question
Is a blocking peptide available for product anti-SKP2 antibody (PA1102)?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We do provide the blocking peptide for product anti-SKP2 antibody (PA1102). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-12
Question
I was wanting to use your anti-SKP2 antibody for IHC for human oocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human oocyte identification?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
You can see on the product datasheet, PA1102 anti-SKP2 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-03
Question
My question regards to test anti-SKP2 antibody PA1102 on human oocyte for research purposes, then I may be interested in using anti-SKP2 antibody PA1102 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
The products we sell, including anti-SKP2 antibody PA1102, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-15
Question
We are currently using anti-SKP2 antibody PA1102 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
G. Zhang
Verified customer
Asked: 2018-12-24
Answer
The anti-SKP2 antibody (PA1102) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-24
Question
Here is the WB image, lot number and protocol we used for oocyte using anti-SKP2 antibody PA1102. Please let me know if you require anything else.
D. Mangal
Verified customer
Asked: 2017-09-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-29
