Anti-p38 MAPK14 Rabbit Monoclonal Antibody

p38 alpha antibody

Boster Bio Anti-p38 MAPK14 Rabbit Monoclonal Antibody catalog # M00176. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 6 publication(s).

Product Info Summary

SKU: M00176
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, ICC, WB

Product Name

Anti-p38 MAPK14 Rabbit Monoclonal Antibody

View all p38 alpha Antibodies

SKU/Catalog Number

M00176

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-p38 MAPK14 Rabbit Monoclonal Antibody catalog # M00176. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p38 MAPK14 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00176)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DFG-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human p38

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00176 is reactive to MAPK14 in Human, Mouse, Rat

Applications

M00176 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

41.293kDa

Background of p38 alpha

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:100-1:500
IF
1:100
FC 1:150

Validation Images & Assay Conditions

Gene/Protein Information For MAPK14 (Source: Uniprot.org, NCBI)

Gene Name

MAPK14

Full Name

Mitogen-activated protein kinase 14

Weight

41.293kDa

Superfamily

protein kinase superfamily

Alternative Names

MAPK14; p38 alpha; SAPK2a MAPK14 CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA mitogen-activated protein kinase 14 mitogen-activated protein kinase 14|CSAID-binding protein|MAP kinase 14|MAP kinase Mxi2|MAP kinase p38 alpha|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|mitogen-activated protein kinase p38 alpha|p38 MAP kinase|p38 mitogen activated protein kinase|p38alpha Exip|stress-activated protein kinase 2A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAPK14, check out the MAPK14 Infographic

MAPK14 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAPK14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00176 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Placental growth factor gene silencing mitigates the epithelial‑to‑mesenchymal transition via the p38 MAPK pathway in rats with hyperoxia‑induced lung injury

Wei X,Zhu Q,Liu N,Xu L,Wei S,Fan Z,Sun C,Zhao Y,Qiao M,Wu J,Hu D,Wang Y,Sun P.Neuroprotective Effects and Mechanisms of Zhenlong Xingnao Capsule in In Vivo and In Vitro Models of Hypoxia.Front Pharmacol.2019 Sep 26;10:1096.doi:10.3389/fphar.2019.01096.PMID:31611791;PMCID:PMC6775503.
Species: Rat
M00176 usage in article: APP:IHC, SAMPLE:CEREBRAL TISSUE, DILUTION:NA

Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14
Species: Mouse
M00176 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA

Neuroprotective Effects and Mechanisms of Zhenlong Xingnao Capsule in In Vivo and In Vitro Models of Hypoxia Xia Wei 1, Qingfen Zhu 1, Na Liu 1, Lihua Xu 1, Sheng Wei 2, Zhiyun Fan 1, Changhua Sun 1, Yan Zhao 1, Mingqi Qiao 2, Jibiao Wu 2, Defu Hu 1, Ya

Vasopressin decreases neuronal apoptosis during cardiopulmonary resuscitation

Liao S, Xiao S, Chen H, Zhang M, Chen Z, Long Y, Gao L, He J, Ge Y, Yi W, Wu M, Li G, Zhou Y. Int J Oncol. 2017 Nov;51(5):1497-1507. doi: 10.3892/ijo.2017.4137. Epub 2017 Sep 27. The receptor for activated protein kinase C promotes cell growth, in...

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5 Customer Q&As for Anti-p38 MAPK14 Rabbit Monoclonal Antibody

Question

We have observed staining in human hepatoma. Are there any suggestions? Is anti-p38 Rabbit Monoclonal antibody supposed to stain hepatoma positively?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

Based on literature hepatoma does express MAPK14. Based on Uniprot.org, MAPK14 is expressed in blood, peripheral blood, liver, b-cell, renal cell carcinoma, placenta, platelet, hepatoma, embryonic kidney, cervix carcinoma, leukemic t-cell, t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MAPK14 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 10727080
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Hepatoma, Pubmed ID: 10943842
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 7696354, 24275569
Peripheral blood, Pubmed ID: 7997261
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801, 18088087
Renal cell carcinoma, Pubmed ID: 11866441
T-cell, Pubmed ID: 21586573

Boster Scientific Support

Answered: 2020-04-01

Question

My team were happy with the WB result of your anti-p38 Rabbit Monoclonal antibody. However we have seen positive staining in t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is MAPK14 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

Based on literature, t-cell does express MAPK14. Generally MAPK14 expresses in cytoplasm. Regarding which tissues have MAPK14 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 10727080
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Hepatoma, Pubmed ID: 10943842
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 7696354, 24275569
Peripheral blood, Pubmed ID: 7997261
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801, 18088087
Renal cell carcinoma, Pubmed ID: 11866441
T-cell, Pubmed ID: 21586573

Boster Scientific Support

Answered: 2019-10-15

Question

Our lab used your anti-p38 Rabbit Monoclonal antibody for WB on cervix carcinoma a few months ago. I am using mouse, and We are going to use the antibody for IF next. you antibody examining cervix carcinoma as well as b-cell in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

I took a look at the website and datasheets of our anti-p38 Rabbit Monoclonal antibody and I see that M00176 has been validated on mouse in both WB and IF. Thus M00176 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-10

Question

We are currently using anti-p38 Rabbit Monoclonal antibody M00176 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

The anti-p38 Rabbit Monoclonal antibody (M00176) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-18

Question

We want using your anti-p38 Rabbit Monoclonal antibody for p38mapk events studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

D. Carter

Verified customer

Asked: 2018-07-24

Answer

We appreciate your inquiry. This M00176 anti-p38 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-07-24

Order DetailsPrice
M00176

100μL

$370
M00176-30ul

30μL sample

$99
M00176-carrier-free

Carrier Free

$370

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