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- Table of Contents
SQSTM1 antibody (M00300) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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Immunofluorescent analysis of Hela cells, using p62/SQSTM1 Antibody.
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Anti-p62/SQSTM1 Rabbit Monoclonal Antibody
SQSTM1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
SQSTM1 antibody was positively detected by WB in:
human HepG2 whole cell, human A549 whole cell, human RT4 whole cell, human Hela whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SQSTM1 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human spleen tissue, human cervix squamous cell carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-p62/SQSTM1 Rabbit Monoclonal Antibody
View all SQSTM1 Antibodies
View all SQSTM1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EBB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p62/SQSTM1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
62 kDa
Calculated Molecular Weight
47.7 kDa
Cite This Product
Anti-p62/SQSTM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00300)
Protocols
Western Blot protocol for SQSTM1 antibody
Immunohistochemistry (IHC) protocol for SQSTM1 antibody
Publications
Application:
Species:
Ionizing radiation promotes lung injury by inducing ferroptosis-driven senescence in epithelial cells via NCOA4-mediated ferritinophagy
Published: 2026-02-18
Journal: Redox Biology
Application: WB
Impact Factor: 11.90
Species: Human
PMID: 41722510
Hydrogen alleviates cardiac ferroptosis damage under chronic intermittent hypoxia conditions by targeting Nrf2
Published: 2025-12-09
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Mouse
PMID: 41370977
Fu's Subcutaneous Needling Therapy Significantly Enhances Pain Relief in Sciatica Rats by Promoting Mitochondrial Autophagy
Published: 2025-10-31
Journal: Biomedical Journal
Application: IHC,IF
Impact Factor: 4.40
Species: Rat
PMID: 41177207
Collagen type I alpha 1 induces radio-resistance in hypoxic glioblastoma cells by promoting autophagy
Published: 2025-08-12
Journal: JOURNAL OF NEURO-ONCOLOGY
Application: WB
Impact Factor: 3.10
Species: Human
PMID: 40794247
GLDC attenuates liver ischemia-reperfusion injury by inhibiting macrophage recruitment and activation via PTBP1/P2RY6
Published: 2025-07-03
Journal: CELLULAR SIGNALLING
Application: WB
Impact Factor: 3.70
Species: Rat
PMID: 40617371
Polyphyllin I Inhibits the Metastasis of Cervical Cancer Through the Regulation of the β-Catenin Signaling Pathway
Published: 2025-05-12
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 4.90
Species: Human
PMID: 40429774
Salidroside Derivative SHPL-49 Exerts Anti-Neuroinflammatory Effects by Modulating Excessive Autophagy in Microglia
Published: 2025-03-13
Journal: Cells
Application: IF,WB
Impact Factor: 5.20
Species: Mouse
PMID: 40136674
Phillygenin rescues impaired autophagy flux by modulating the PI3K/Akt/mToR signaling pathway in a rat model of severe acute pancreatitis
Published: 2024-12-17
Journal: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY
Application: IHC
Impact Factor: 3.00
Species: Rat
PMID: 39688211
Mitoxantrone ameliorates ineffective erythropoiesis in a β-thalassemia intermedia mouse model
Published: 2024-06-11
Journal: Blood Advances
Application: WB
Impact Factor: 7.40
Species: Mouse
PMID: 38861356
Lower dose of metformin combined with artesunate induced autophagy-dependent apoptosis of glioblastoma by activating ROS-AMPK-mTOR axis
Published: 2023-07-01
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.70
Species: Human
PMID: 37399981
Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy
Published: 2020-12-04
Journal: Frontiers in Oncology
Impact Factor: 4.85
PMID: 33344232
Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction
Published: 2019-08-22
Journal: Cell Communication and Signaling
Application: WB
Impact Factor: 5.11
Species: Human
PMID: 31438969
Review
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Product Promise
We promise Anti-p62/SQSTM1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00300
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026