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Product Info Summary
| SKU: | A01726 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Parafibromin CDC73 Antibody
SKU/Catalog Number
A01726
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Parafibromin CDC73 Antibody catalog # A01726. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Parafibromin CDC73 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01726)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Parafibromin. at AA range: 51-100
Reactive Species
A01726 is reactive to CDC73 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
60.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01726 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ELISA 1:10000-20000
Validation Images & Assay Conditions
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Western Blot analysis of 1) mouse heart, 2) mouse spleen cells using primary antibody diluted at 1: 500 (4℃ overnight) . Secondary antibody: Goat Anti-rabbit IgG IRDye 800 (diluted at 1: 5000, 25℃, 1 hour).
Specific Publications For Anti-Parafibromin CDC73 Antibody (A01726)
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Customer Q&As
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6 Customer Q&As for Anti-Parafibromin CDC73 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Parafibromin antibody A01726. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-26
Question
Would anti-Parafibromin antibody A01726 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
According to the expression profile of erythroleukemia, CDC73 is highly expressed in erythroleukemia. So, it is likely that anti-Parafibromin antibody A01726 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2019-07-23
Question
Is a blocking peptide available for product anti-Parafibromin antibody (A01726)?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We do provide the blocking peptide for product anti-Parafibromin antibody (A01726). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-28
Question
I see that the anti-Parafibromin antibody A01726 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-01-17
Question
My question regarding product A01726, anti-Parafibromin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Roberts
Verified customer
Asked: 2018-08-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01726 anti-Parafibromin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-16
Question
Does A01726 anti-Parafibromin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Wu
Verified customer
Asked: 2015-06-10
Answer
As indicated on the product datasheet, A01726 anti-Parafibromin antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-06-10
