Anti-Aquaporin 0/MIP Antibody Picoband®

Product Name

Anti-Aquaporin 0/MIP Antibody Picoband®

View all Mip Antibodies

SKU/Catalog Number

PA2110

Size

100 μg/vial

Form

Lyophilized

Description

Pathway/function: water transport via lens fiber channel AQP0/MIP (putative; GO:0006833) supporting lens homeostasis and transparency; additional roles in cell–cell adhesion (putative). Assay context: rabbit polyclonal primary validated for Western blot; reactive species human/mouse/rat (per product page). In membrane-protein panels, may be contrasted with sperm-specific metalloprotease ADAM2 to highlight tissue-restricted expression (putative).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aquaporin 0/MIP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2110)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aquaporin 0, identical to the related rat sequence, and different from the related human sequence by two amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2110 is reactive to Mip in Human, Mouse, Rat

Observed Molecular Weight

50 kDa

Calculated molecular weight

27.9 kDa

Background of Mip

Lens fiber major intrinsic protein also called MIP26 or MP26 is a protein that in humans is encoded by the MIP gene. MIP is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. Using 2-color fluorescence in situ hybridization on high-resolution R-banded chromosomes and human genomic DNA clones for MIP as probes, this gene was found that located in close proximity in region 12q13. MIP plays a crucial role in the development of a transparent eye lens. This gene may be responsible for regulating the osmolarity of the lens and interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2110 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Human, Rat

Positive Control

WB: Mouse Spleen Tissue, Mouse Intestine Tissue

Validation Images & Assay Conditions

See full target information Mip

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