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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Human PD-1 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human PD-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PDCD1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human PD-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0959)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L25-Q167
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0959 is reactive to PDCD1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK0959 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0959-CAP||Anti-Human PDCD1 Pre-coated 96-well strip microplate||1|
|EK0959-ST||Human PDCD1 Standard||2 vials, 10 ng/tube|
|EK0959-DA||Human PDCD1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human PD-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PD-1, Dilution ratio of 1:1, concentration in serum is 80-120pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PD-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of PD-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For PDCD1 (Source: Uniprot.Org, NCBI)
Programmed cell death protein 1
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2 PDCD1 CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1 programmed cell death 1 programmed cell death protein 1|programmed cell death 1 protein|protein PD-1|systemic lupus erythematosus susceptibility 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PDCD1, check out the PDCD1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PDCD1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK0959
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