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Product Info Summary
| SKU: | M01429-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Cow, Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F, ICC |
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Product info
Product Name
Anti-Perforin Purified PRF1 Monoclonal Antibody
SKU/Catalog Number
M01429-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Perforin Purified PRF1 Monoclonal Antibody (Catalog# M01429-1). Tested in Flow Cytometry, IP, IHC-P, IHC-F, ICC application(s). This antibody reacts with Human, Cow.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Perforin Purified PRF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01429-1)
Host
Mouse
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
dG9
Isotype
Mouse IgG2b kappa
Immunogen
purified granules from human YT lymphoma cell line. The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
Reactive Species
M01429-1 is reactive to PRF1 in Cow, Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
61.4 kDa
Background of PRF1
Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01429-1 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 3-12 μg/ml. Intracellular staining.
Validation Images & Assay Conditions
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Separation of perforin positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracelluar staining) of human peripheral whole blood using anti-human perforin (dG9) purified antibody (concentration in sample 2 µg/ml, GAM FITC).
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Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Perforin (dG9) purified antibody (concentration in sample 2 µg/ml, GAM FITC).
Specific Publications For Anti-Perforin Purified PRF1 Monoclonal Antibody (M01429-1)
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