Anti-Perforin/PRF1 Antibody Picoband™

Perforin antibody

Boster Bio Anti-Perforin/PRF1 Antibody Picoband™ catalog # PB9155. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB9155
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Perforin/PRF1 Antibody Picoband™

View all Perforin Antibodies

SKU/Catalog Number

PB9155

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Perforin/PRF1 Antibody Picoband™ catalog # PB9155. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Perforin/PRF1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9155)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Perforin recombinant protein (Position: E175-W555). Human Perforin shares 68% amino acid (aa) sequence identity with both mouse and rat Perforin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9155 is reactive to PRF1 in Human

Applications

PB9155 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

61 kDa

Calculated molecular weight

61.377kDa

Background of Perforin

PRF1, also known as Perforin-1, is a protein that in humans is encoded by the PRF1 gene. It is mapped to 10q22.1. PRF1 is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, PRF1 inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. PRF1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. PRF1 is thought to act by creating holes in the plasma membrane which triggers an influx of calcium and initiates membrane repair mechanisms. These repair mechanisms bring perforin and granzymes into early endosomes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For PRF1 (Source: Uniprot.org, NCBI)

Gene Name

PRF1

Full Name

Perforin-1

Weight

61.377kDa

Superfamily

complement C6/C7/C8/C9 family

Alternative Names

Cytolysin; FLH2; HPLH2; HPLH2lymphocyte pore forming protein; Lymphocyte pore-forming protein; MGC65093; P1; P1PFN1; perforin 1 (pore forming protein); Perforin; perforin-1; PFP; PFPcytolysin; PRF1 PRF1 HPLH2, P1, PFP perforin 1 perforin-1|cytolysin|lymphocyte pore-forming protein|perforin 1 (pore forming protein)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRF1, check out the PRF1 Infographic

PRF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Perforin/PRF1 Antibody Picoband™

Question

My boss were satisfied with the WB result of your anti-Perforin/PRF1 antibody. However we have seen positive staining in natural killer cell cytoplasmic granule lumen. secreted. cell using this antibody. Is that expected? Could you tell me where is PRF1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

According to literature, natural killer cell does express PRF1. Generally PRF1 expresses in cytoplasmic granule lumen. secreted. cell. Regarding which tissues have PRF1 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Natural killer cell, Pubmed ID: 3419519, 8676885
Spleen, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-10-15

Question

We are currently using anti-Perforin/PRF1 antibody PB9155 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-06-15

Answer

The anti-Perforin/PRF1 antibody (PB9155) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-06-15

Question

We have seen staining in human spleen. Any tips? Is anti-Perforin/PRF1 antibody supposed to stain spleen positively?

Verified Customer

Verified customer

Asked: 2018-05-17

Answer

From literature spleen does express PRF1. From Uniprot.org, PRF1 is expressed in mononuclear cell, natural killer cell, spleen, liver, among other tissues. Regarding which tissues have PRF1 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Natural killer cell, Pubmed ID: 3419519, 8676885
Spleen, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-05-17

Question

you antibody using your anti-Perforin/PRF1 antibody for t cell mediated cytotoxicity studies. Has this antibody been tested with western blotting on colo320 whole cell lysate? We would like to see some validation images before ordering.

N. Krishna

Verified customer

Asked: 2015-08-14

Answer

I appreciate your inquiry. This PB9155 anti-Perforin/PRF1 antibody is validated on hela whole cell lysate, colo320 whole cell lysate, hepg2 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-08-14

Order DetailsPrice
PB9155

100μg

$370
PB9155-10ug

10μg sample (liquid)

$99
PB9155-Biotin

100 μg Biotin conjugated

$570
PB9155-Cy3

100 μg Cy3 conjugated

$570
PB9155-Dylight488

100 μg Dylight488 conjugated

$570
PB9155-Dylight550

100 μg Dylight550 conjugated

$570
PB9155-Dylight594

100 μg Dylight594 conjugated

$570
PB9155-FITC

100 μg FITC conjugated

$570
PB9155-HRP

100 μg HRP conjugated

$570
PB9155-APC

100 μg APC conjugated

$670
PB9155-PE

100 μg PE conjugated

$670
PB9155-carrier-free

Carrier Free

$370

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