Anti-PERK EIF2AK3 Antibody

Boster Bio Anti-PERK EIF2AK3 Antibody catalog # A01992. Tested in IP, IHC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: A01992
Size: 100μL
Reactive Species: Human
Host: Rabbit
Application: IP, IHC, WB

Product Name

Anti-PERK EIF2AK3 Antibody

See all PERK products

SKU/Catalog Number

A01992

Size

100μL

Form

Liquid

Description

Boster Bio Anti-PERK EIF2AK3 Antibody catalog # A01992. Tested in IP, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PERK EIF2AK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01992)

Host

Rabbit

Contents

0.02 M Potassium Phosphate
0.15 M Sodium Chloride
pH 7.2
0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK (see link below for the full length sequence of the mouse gene product).

Reactive Species

A01992 is reactive to EIF2AK3 in Human

Applications

A01992 is guaranteed for IP, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blot, 1:500-1:1:3000, mouse
ImmunoPrecipitation: 10-30ul, mouse

Validation Images & Assay Conditions

Gene/Protein Information For EIF2AK3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NZJ5

Gene ID

9451

Gene Name

EIF2AK3

Full Name

Eukaryotic translation initiation factor 2-alpha kinase 3

Weight

125.216kDa

Superfamily

protein kinase superfamily

Alternative Names

DKFZp781H1925; EC 2.7.11.1; EIF2AK3; eukaryotic translation initiation factor 2 alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; hsPEK; PEK; PEKPERKPancreatic eIF2-alpha kinase; PERK; PRKR-like endoplasmic reticulum kinase; WRS EIF2AK3 PEK, PERK, WRS eukaryotic translation initiation factor 2 alpha kinase 3 eukaryotic translation initiation factor 2-alpha kinase 3|PRKR-like endoplasmic reticulum kinase|pancreatic EIF2-alpha kinase|truncated eukaryotic translation initiation factor 2 alpha kinase 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EIF2AK3, check out the EIF2AK3 Infographic

EIF2AK3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EIF2AK3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01992 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress

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16 Customer Q&As for Anti-PERK EIF2AK3 Antibody

Question

We were happy with the WB result of your anti-PERK antibody. However we have observed positive staining in cervix carcinoma endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is EIF2AK3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

From what I have seen in literature, cervix carcinoma does express EIF2AK3. Generally EIF2AK3 expresses in endoplasmic reticulum membrane. Regarding which tissues have EIF2AK3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, and Pancreas, Pubmed ID: 10677345
Cervix carcinoma, Pubmed ID: 20068231
Liver, Pancreas, and Testis, Pubmed ID: 10026192

Boster Scientific Support

Answered: 2020-04-24

Question

I was wanting to use your anti-PERK antibody for IP for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

You can see on the product datasheet, A01992 anti-PERK antibody has been validated for IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-02

Question

Does anti-PERK antibody A01992 work for IP with liver?

Verified Customer

Verified customer

Asked: 2020-02-25

Answer

According to the expression profile of liver, EIF2AK3 is highly expressed in liver. So, it is likely that anti-PERK antibody A01992 will work for IP with liver.

Boster Scientific Support

Answered: 2020-02-25

Question

Is there a BSA free version of anti-PERK antibody A01992 available?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PERK antibody A01992 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-23

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PERK antibody A01992. Let me know if you need anything else.

R. Kulkarni

Verified customer

Asked: 2019-09-27

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-27

Question

My question regarding product A01992, anti-PERK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01992 anti-PERK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-02

Question

We are currently using anti-PERK antibody A01992 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

The anti-PERK antibody (A01992) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-19

Question

I am looking for using your anti-PERK antibody for cellular response to cold studies. Has this antibody been tested with western blotting on 293t cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

Thanks for your inquiry. This A01992 anti-PERK antibody is tested on 293t cell lysate. It is guaranteed to work for IP, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-25

Question

We have tried in the past anti-PERK antibody for IHC on brain pancreas last year. I am using human, and We want to use the antibody for IP next. We are interested in examining brain pancreas as well as nasal cavity mucosa in our next experiment. Could give a recommendation on which antibody would work the best for IP?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

I looked at the website and datasheets of our anti-PERK antibody and it seems that A01992 has been tested on human in both IHC and IP. Thus A01992 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-24

Question

Will A01992 anti-PERK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

It shows on the product datasheet, A01992 anti-PERK antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-19

Question

Is a blocking peptide available for product anti-PERK antibody (A01992)?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

We do provide the blocking peptide for product anti-PERK antibody (A01992). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-27

Question

Is this A01992 anti-PERK antibody reactive to the isotypes of EIF2AK3?

L. Wu

Verified customer

Asked: 2018-10-08

Answer

The immunogen of A01992 anti-PERK antibody is This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK (see link below for the full length sequence of the mouse gene product). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-10-08

Question

We have seen staining in human cervix carcinoma. Any tips? Is anti-PERK antibody supposed to stain cervix carcinoma positively?

N. Jones

Verified customer

Asked: 2017-06-27

Answer

From literature cervix carcinoma does express EIF2AK3. From Uniprot.org, EIF2AK3 is expressed in nasal cavity mucosa, liver, pancreas testis, brain pancreas, brain, cervix carcinoma, among other tissues. Regarding which tissues have EIF2AK3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, and Pancreas, Pubmed ID: 10677345
Cervix carcinoma, Pubmed ID: 20068231
Liver, Pancreas, and Testis, Pubmed ID: 10026192

Boster Scientific Support

Answered: 2017-06-27

Question

I was wanting to use to test anti-PERK antibody A01992 on human liver for research purposes, then I may be interested in using anti-PERK antibody A01992 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Anderson

Verified customer

Asked: 2016-11-16

Answer

The products we sell, including anti-PERK antibody A01992, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-11-16

Question

I see that the anti-PERK antibody A01992 works with IP, what is the protocol used to produce the result images on the product page?

A. Jones

Verified customer

Asked: 2016-06-16

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-06-16

Question

I have attached the WB image, lot number and protocol we used for liver using anti-PERK antibody A01992. Please let me know if you require anything else.

K. Mangal

Verified customer

Asked: 2015-10-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-10-02

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A01992
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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