Product Info Summary
SKU: | M05077 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-AMPK beta 2 PRKAB2 Antibody Picoband™ (monoclonal, 6G1)
View all AMPK beta 2/PRKAB2 Antibodies
SKU/Catalog Number
M05077
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AMPK beta 2 PRKAB2 Antibody Picoband™ (monoclonal, 6G1) catalog # M05077. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMPK beta 2 PRKAB2 Antibody Picoband™ (monoclonal, 6G1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05077)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6G1
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 2, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M05077 is reactive to PRKAB2 in Human
Applications
M05077 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
34 kDa
Calculated molecular weight
30.302kDa
Background of AMPK beta 2/PRKAB2
5'-AMP-activated protein kinase subunit beta-2 is an enzyme that in humans is encoded by the PRKAB2 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry analysis of PC-3 cells using anti-AMPK beta 2 antibody (M05077).
Overlay histogram showing PC-3 cells stained with M05077 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AMPK beta 2 Antibody (M05077,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 2. Western blot analysis of AMPK beta 2 using anti-AMPK beta 2 antibody (M05077).
Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human placenta tissue lysate,
Lane 3: human 293T whole cell lysate,
Lane 4: human A549 whole cell lysate,
Lane 5: human A375 whole cell lysate,
Lane 6: human A431 whole cell lysate,
Lane 7: human U20S whole cell lysate,
Lane 8: human K562 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AMPK beta 2 antigen affinity purified monoclonal antibody (Catalog # M05077) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Protein Target Info & Infographic
Gene/Protein Information For PRKAB2 (Source: Uniprot.org, NCBI)
Gene Name
PRKAB2
Full Name
5'-AMP-activated protein kinase subunit beta-2
Weight
30.302kDa
Superfamily
5'-AMP-activated protein kinase beta subunit family
Alternative Names
5'-AMP-activated protein kinase, beta-2 subunit; AMPK beta 2; AMPK beta 2,5'-AMP-activated protein kinase subunit beta-2; AMPK beta-2 chain; AMPK subunit beta-2; MGC61468; PRKAB2; protein kinase, AMP-activated, beta 2 non-catalytic subunit PRKAB2 protein kinase AMP-activated non-catalytic subunit beta 2 5-AMP-activated protein kinase subunit beta-2|5-AMP-activated protein kinase, beta-2 subunit|AMP-activated protein kinase beta 2 non-catalytic subunit|AMPK beta 2|AMPK beta-2 chain|AMPK subunit beta-2|protein kinase, AMP-activated, beta 2 non-catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAB2, check out the PRKAB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AMPK beta 2 PRKAB2 Antibody Picoband™ (monoclonal, 6G1) (M05077)
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No publications found for M05077
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