Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband™

Peroxiredoxin 3 antibody

Boster Bio Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband™ catalog # PB9349. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9349
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband™

View all Peroxiredoxin 3 Antibodies

SKU/Catalog Number

PB9349

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband™ catalog # PB9349. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9349)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Peroxiredoxin 3 recombinant protein (Position: T110-Q256). Human Peroxiredoxin 3 shares 93% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9349 is reactive to PRDX3 in Human, Mouse, Rat

Applications

PB9349 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

27.693kDa

Background of Peroxiredoxin 3

PRDX3 (peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86% amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For PRDX3 (Source: Uniprot.org, NCBI)

Gene Name

PRDX3

Full Name

Thioredoxin-dependent peroxide reductase, mitochondrial

Weight

27.693kDa

Superfamily

peroxiredoxin family

Alternative Names

Antioxidant protein 1MGC104387; AOP-1; AOP-1MGC24293; AOP1PRO1748; EC 1.11.1.15; HBC189; MER5; Peroxiredoxin 3; Peroxiredoxin III; peroxiredoxin-3; PRDX3; Protein MER5 homolog; prx-III; SP-22; thioredoxin-dependent peroxide reductase, mitochondrial PRDX3 AOP-1, AOP1, HBC189, MER5, PRO1748, SP-22, prx-III peroxiredoxin 3 thioredoxin-dependent peroxide reductase, mitochondrial|antioxidant protein 1|epididymis secretory sperm binding protein|peroxiredoxin III|protein MER5 homolog|thioredoxin-dependent peroxiredoxin 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRDX3, check out the PRDX3 Infographic

PRDX3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRDX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband™

Question

We are currently using anti-Peroxiredoxin 3/PRDX3 antibody PB9349 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

The anti-Peroxiredoxin 3/PRDX3 antibody (PB9349) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-03

Order DetailsPrice
PB9349

100μg

$370
PB9349-10ug

10μg sample (liquid)

$99
PB9349-Biotin

100 μg Biotin conjugated

$570
PB9349-Cy3

100 μg Cy3 conjugated

$570
PB9349-Dylight488

100 μg Dylight488 conjugated

$570
PB9349-Dylight550

100 μg Dylight550 conjugated

$570
PB9349-Dylight594

100 μg Dylight594 conjugated

$570
PB9349-FITC

100 μg FITC conjugated

$570
PB9349-HRP

100 μg HRP conjugated

$570
PB9349-APC

100 μg APC conjugated

$670
PB9349-PE

100 μg PE conjugated

$670
PB9349-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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