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- Table of Contents
SEMA3A antibody (PB9353) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Anti-Semaphorin 3A Picoband antibody, PB9353, Western blotting
All lanes: Anti Semaphorin 3A (PB9353) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 89KD
Observed bind size: 89KD
All lanes: Anti Semaphorin 3A (PB9353) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 89KD
Observed bind size: 89KD
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Anti-Semaphorin 3A/SEMA3A Antibody Picoband®
SEMA3A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
SEMA3A antibody was positively detected by WB in:
293T Whole Cell, A549 Whole Cell, HELA Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Semaphorin 3A/SEMA3A Antibody Picoband®
View all SEMA3A Antibodies
View all SEMA3A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Semaphorin 3A recombinant protein (Position: K550-V771). Human Semaphorin 3A shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Semaphorin 3A, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
89 kDa
Calculated Molecular Weight
88.9 kDa
Background of SEMA3A
SEMA3A is also known as COLL1, SEMA1 or SEMAL. This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
Cite This Product
Anti-Semaphorin 3A/SEMA3A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9353)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Sheep
97%
High Homology
Cow
97%
High Homology
Dog
97%
High Homology
Rat
95%
High Homology
Mouse
93%
High Homology
Rabbit
82%
Moderate Homology
Zebrafish
64%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SEMA3A antibody
Publications
Application:
Species:
Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A
Published: 2018-10-01
Journal: International Journal of Clinical and Experimental Pathology
Application: WB
Impact Factor: 1.40
Species: Human
PMID: 31949563
Significance of semaphorin‑3A and MMP‑14 protein expression in non‑small cell lung cancer
Published: 2014-02-27
Journal: Oncology Letters
Application: IHC
Impact Factor: 0.99
Species: Human
PMID: 24765144
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Product Promise
We promise Anti-Semaphorin 3A/SEMA3A Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9353
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026