Anti-Semaphorin 3A/SEMA3A Antibody Picoband®

Product Name

Anti-Semaphorin 3A/SEMA3A Antibody Picoband®

View all SEMA3A Antibodies

SKU/Catalog Number

PB9353
PB0370 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Semaphorin 3A/SEMA3A Antibody Picoband® catalog # PB9353. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Semaphorin 3A/SEMA3A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9353)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Semaphorin 3A recombinant protein (Position: K550-V771). Human Semaphorin 3A shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Semaphorin 3A, respectively.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9353 is reactive to SEMA3A in Human

Observed Molecular Weight

89 kDa

Calculated molecular weight

88.9 kDa

Background of SEMA3A

SEMA3A is also known as COLL1, SEMA1 or SEMAL. This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9353 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: 293T Whole Cell, A549 Whole Cell, HELA Whole Cell

Validation Images & Assay Conditions

See full target information SEMA3A

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