Product Info Summary
| SKU: | P00994-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-AMPK Alpha 1/PRKAA1 (S486) Antibody
SKU/Catalog Number
P00994-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-AMPK Alpha 1/PRKAA1 (S486) Antibody catalog # P00994-2. Tested in WB, IHC, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-Phospho-AMPK Alpha 1/PRKAA1 (S486) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00994-2)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
A synthesized peptide derived from human AMPK alpha 1 around the phosphorylation site of Ser486.
Reactive Species
P00994-2 is reactive to PRKAA1 in Human, Mouse, Rat
Calculated molecular weight
64.0 kDa
Background of PRKAA1
5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00994-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Specific Publications For Anti-Phospho-AMPK Alpha 1/PRKAA1 (S486) Antibody (P00994-2)
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