Product Info Summary
SKU: | A01600S63 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-ATF-1 (S63) Antibody
SKU/Catalog Number
A01600S63
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-ATF-1 (S63) Antibody catalog # A01600S63. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-ATF-1 (S63) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01600S63)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ATF-1 around the phosphorylation site of S63.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01600S63 is reactive to ATF1 in Human, Mouse
Applications
A01600S63 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
29.233kDa
Background of ATF1
Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.
Ouyang P., J. Cell Biol. 135:1027-1042(1996).
Degen W.G.J., Biochim. Biophys. Acta 1444-384-394(1999).
Shi Y., Oncogene 19:289-297(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:5000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Phospho-ATF-1 (S63) Antibody A01600S63
Western Blot (WB) analysis of Mouse Kidney KB 293T 3T3 lysis using Phospho-ATF-1 (S63) antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ATF1 (Source: Uniprot.org, NCBI)
Gene Name
ATF1
Full Name
Cyclic AMP-dependent transcription factor ATF-1
Weight
29.233kDa
Superfamily
bZIP family
Alternative Names
activating transcription factor 1FUS/ATF-1; ATF1; cAMP-dependent transcription factor ATF-1; EWS-ATF1; Protein TREB36; TREB36; TREB36cyclic AMP-dependent transcription factor ATF-1 ATF1 EWS-ATF1, FUS/ATF-1, TREB36 activating transcription factor 1 cyclic AMP-dependent transcription factor ATF-1|cAMP-dependent transcription factor ATF-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF1, check out the ATF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-ATF-1 (S63) Antibody (A01600S63)
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No publications found for A01600S63
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