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Product Info Summary
| SKU: | P00080 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-Caspase 9/CASP9 (S196) Antibody
SKU/Catalog Number
P00080
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-Caspase 9/CASP9 (S196) Antibody catalog # P00080. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-Phospho-Caspase 9/CASP9 (S196) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00080)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
A synthesized peptide derived from human Caspase 9 around the phosphorylation site of Ser196.
Reactive Species
P00080 is reactive to CASP9 in Human, Mouse
Calculated molecular weight
46.3 kDa
Background of CASP9
CASP9 is also known as MCH6 or APAF3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00080 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Validation Images & Assay Conditions
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Specific Publications For Anti-Phospho-Caspase 9/CASP9 (S196) Antibody (P00080)
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