Anti-Phospho-Cyclin D1/CCND1 (S90) Antibody

Product Name

Anti-Phospho-Cyclin D1/CCND1 (S90) Antibody

View all CCND1 Antibodies

SKU/Catalog Number

P00149-1

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-Phospho-Cyclin D1/CCND1 (S90) Antibody catalog # P00149-1. Tested in WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Cite This Product

Anti-Phospho-Cyclin D1/CCND1 (S90) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00149-1)

Host

Rabbit

Contents

500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Immunogen

A synthesized peptide derived from human Cyclin D1 around the phosphorylation site of Ser90.

Reactive Species

P00149-1 is reactive to CCND1 in Human, Mouse, Rat

Calculated molecular weight

33.7 kDa

Background of CCND1

Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin induced IGF2 mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P00149-1 is guaranteed for IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunocytochemistry/Immunofluorescence, 1:50-400

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information CCND1

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