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Product Info Summary
| SKU: | P02199-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-G3BP1 (S232) Antibody
SKU/Catalog Number
P02199-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-G3BP1 (S232) Antibody catalog # P02199-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-G3BP1 (S232) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02199-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human G3BP-1 around the phosphorylation site of Ser232. AA range:216-248
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P02199-1 is reactive to G3BP1 in Human, Mouse
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P02199-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-G3BP1 (S232) Polyclonal Antibody
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Western blot analysis of extracts from HeLa cells, using G3BP-1 (Phospho-Ser232) Antibody. The lane on the right is treated with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast cancer, using G3BP-1 (Phospho-Ser232) Antibody. The picture on the right is blocked with the G3BP-1 (Phospho-Ser232) peptide.
Specific Publications For Anti-Phospho-G3BP1 (S232) Antibody (P02199-1)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Phospho-G3BP1 (S232) Antibody
Question
Will P02199-1 anti-Phospho-G3BP1 (S232) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
As indicated on the product datasheet, P02199-1 anti-Phospho-G3BP1 (S232) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-24
Question
Is this P02199-1 anti-Phospho-G3BP1 (S232) antibody reactive to the isotypes of G3BP1?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The immunogen of P02199-1 anti-Phospho-G3BP1 (S232) antibody is Synthesized peptide derived from human G3BP1 around the phosphorylation site of S232. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-09
Question
We are currently using anti-Phospho-G3BP1 (S232) antibody P02199-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on primate tissues as well?
T. Singh
Verified customer
Asked: 2018-12-31
Answer
The anti-Phospho-G3BP1 (S232) antibody (P02199-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-31
