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Product Info Summary
| SKU: | P01504 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Hexokinase 1/HK1 (Y732) Antibody
SKU/Catalog Number
P01504
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-Hexokinase 1/HK1 (Y732) Antibody catalog # P01504. Tested in WB, IHC, ICC, IF applications. This antibody reacts with Human, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-Phospho-Hexokinase 1/HK1 (Y732) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01504)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
A synthesized peptide derived from human Hexokinase 1 around the phosphorylation site of Tyr732.
Reactive Species
P01504 is reactive to HK1 in Human, Rat
Calculated molecular weight
102.5 kDa
Background of HK1
Hexokinase-1 (HK1) is an enzyme that in humans is encoded by the HK1 gene on chromosome 10. It is mapped to 10q22.1. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01504 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
Validation Images & Assay Conditions
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Specific Publications For Anti-Phospho-Hexokinase 1/HK1 (Y732) Antibody (P01504)
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