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- Table of Contents
Designing a Western blot for Hexokinase-1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the HK1 Western Blot Guide1 Citations
1 Citations
Facts about Hexokinase-1.
| Human | |
|---|---|
| Gene Name: | HK1 |
| Uniprot: | P19367 |
| Entrez: | 3098 |

| Belongs to: |
|---|
| hexokinase family |

Brain form hexokinase; EC 2.7.1; EC 2.7.1.1; glycolytic enzyme; Hexokinase 1; Hexokinase type I; hexokinase-1; HK I; HK1; HK1-ta; HK1-tb; HK1-tc; HKI; HXK1
Mass (kDA):
102.486 kDA

| Human | |
|---|---|
| Location: | 10q22.1 |
| Sequence: | 10; NC_000010.11 (69269991..69401882) |
Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasm, cytosol. The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331).






PMID: 3207429 by Nishi S., et al. Human hexokinase: sequences of amino- and carboxyl-terminal halves are homologous.
PMID: 9531504 by Ruzzo A., et al. Structure of the human hexokinase type I gene and nucleotide sequence of the 5' flanking region.
*More publications can be found for each product on its corresponding product page