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Product Info Summary
| SKU: | P12718-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Phospho-Histone H2B (Ser14) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM238
SKU/Catalog Number
P12718-1
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H2B (Ser14) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM238 (Catalog # P12718-1). Tested in WB, ICC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H2B (Ser14) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM238 (Boster Biological Technology, Pleasanton CA, USA, Catalog # P12718-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM238
Isotype
Rabbit IgG
Immunogen
A phospho-peptide corresponding to Phospho-Histone H2B (Ser14).
Cross-reactivity
This antibody reacts to Histone H2B only when phosphorylated at serine 14. No cross-reactivity with other phosphorylated histones.
Reactive Species
P12718-1 is reactive to HIST1H2BB in Human, Vertebrates
Calculated molecular weight
14.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P12718-1 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5 ug/mL - 2 ug/mL
ICC: 1 μg/mL - 2 μg/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.1 ug/mL – 1 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM238 at 0.5 ug/mL, showed a band of Histone H2B phosphorylated at Serine 14 in HeLa cells.
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ICC result
Immunocytochemistry of HeLa cells using Anti-Phospho-Histone H2B (Ser14) Rabbit mAb RM238 (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green).
Specific Publications For Anti-Phospho-Histone H2B (Ser14) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM238 (P12718-1)
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1 Customer Q&As for Anti-Phospho-Histone H2B (Ser14) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM238
Question
We are currently using anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal antibody, Clone#RM238 P12718-1 for rat tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
M. Huang
Verified customer
Asked: 2018-03-23
Answer
The anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal antibody, Clone#RM238 (P12718-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-23
