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Product Info Summary
| SKU: | P12718 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody
SKU/Catalog Number
P12718
BM4597 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody catalog # P12718. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P12718)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H2B
Reactive Species
P12718 is reactive to HIST1H2BB in Human, Mouse, Rat
Observed Molecular Weight
15 kDa
Calculated molecular weight
14.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P12718 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:10
FC 1:10
Positive Control
WB: Hela cell
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of Histone H2B (acetyl K20) expression in Hela cell treated with TSA lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon, using Histone H2B (acetyl K20) Antibody.
Specific Publications For Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody (P12718)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for lung using anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody P12718. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-16
Question
Would anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody P12718 work for IF with lung?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
According to the expression profile of lung, HIST1H2BB is highly expressed in lung. So, it is likely that anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody P12718 will work for IF with lung.
Boster Scientific Support
Answered: 2020-02-05
Question
I have a question about product P12718, anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P12718 anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-25
Question
I see that the anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody P12718 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-18
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-03-18
