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Product Info Summary
| SKU: | A33947S28 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-Histone H3 (S28) Antibody
SKU/Catalog Number
A33947S28
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H3 (S28) Antibody catalog # A33947S28. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H3 (S28) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A33947S28)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H3 around the phosphorylation site of Ser28. AA range:13-62
Reactive Species
A33947S28 is reactive to H3C1 in Human, Mouse, Rat
Calculated molecular weight
25.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A33947S28 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-Histone H3 (S28) Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western Blot analysis of 293T cells using Phospho-Histone H3 (S28) Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using Histone H3 (Phospho-Ser28) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho-Histone H3 (S28) Antibody (A33947S28)
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