Product Info Summary
SKU: | A01941S212 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC |
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Product info
Product Name
Anti-Phospho-HtrA2 (S212) Antibody
SKU/Catalog Number
A01941S212
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-HtrA2 (S212) Antibody catalog # A01941S212. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-HtrA2 (S212) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01941S212)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human HtrA2 around the phosphorylation site of S212.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01941S212 is reactive to HTRA2 in Human, Mouse, Rat
Applications
A01941S212 is guaranteed for ELISA, IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
48.841kDa
Background of HTRA2/Omi
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Cullin 5 may form a cell surface vasopressin receptor. Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1; elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL; elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kDa protein and adenovirus type 5 E4-orf6. Cullin 5 Interacts with RBX1 and RNF7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Protein Target Info & Infographic
Gene/Protein Information For HTRA2 (Source: Uniprot.org, NCBI)
Gene Name
HTRA2
Full Name
Serine protease HTRA2, mitochondrial
Weight
48.841kDa
Superfamily
peptidase S1C family
Alternative Names
High temperature requirement protein A2; HtrA serine peptidase 2; HTRA2; HtrA-like serine protease; Omi; OMIOmi stress-regulated endoprotease; PARK13EC 3.4.21.108; PRSS25; PRSS25serine, 25; Serine protease 25; serine protease HTRA2, mitochondrial; Serine proteinase OMI HTRA2 MGCA8, OMI, PARK13, PRSS25 HtrA serine peptidase 2 serine protease HTRA2, mitochondrial|HtrA-like serine protease|Omi stress-regulated endoprotease|epididymis secretory sperm binding protein|high temperature requirement protein A2|protease, serine, 25|serine protease 25|serine proteinase OMI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HTRA2, check out the HTRA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HTRA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-HtrA2 (S212) Antibody (A01941S212)
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