Anti-PUMA/BBC3 Antibody

Boster Bio Anti-PUMA/BBC3 Antibody catalog # PA1313. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PA1313
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-PUMA/BBC3 Antibody

See all PUMA products

SKU/Catalog Number

PA1313

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PUMA/BBC3 Antibody catalog # PA1313. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PUMA/BBC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1313)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA (145-159aa ADDLNAQYERRRQEE), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1313 is reactive to BBC3 in Human, Mouse, Rat

Applications

PA1313 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For BBC3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9BXH1

Gene ID

27113

Gene Name

BBC3

Full Name

Bcl-2-binding component 3, isoforms 1/2

Weight

20.532kDa

Superfamily

Bcl-2 family

Alternative Names

BCL2 binding component 3; bcl-2-binding component 3; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMAFLJ42994 BBC3 JFY-1, JFY1, PUMA BCL2 binding component 3 bcl-2-binding component 3|p53 up-regulated modulator of apoptosis|p53-upregulated modulator of apoptosis

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BBC3, check out the BBC3 Infographic

BBC3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BBC3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1313 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Nerve growth factor reduces apoptotic cell death in rat facial motor neurons after facial nerve injury

Zhao Y, Wu S, Wu J, Jia P, Gao S, Yan X, Wang Y. Cancer Biol Ther. 2011 Jan 1;11(1):95-107. Doi: 10.4161/Cbt.11.1.13960. Introduction Of Hypoxia-Targeting P53 Fusion Protein For The Selective Therapy Of Non-Small Cell Lung Cancer.

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3 Customer Q&As for Anti-PUMA/BBC3 Antibody

Question

We are currently using anti-PUMA/BBC3 antibody PA1313 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

The anti-PUMA/BBC3 antibody (PA1313) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-28

Question

Is this PA1313 anti-PUMA/BBC3 antibody reactive to the isotypes of BBC3?

P. Krishna

Verified customer

Asked: 2015-02-16

Answer

The immunogen of PA1313 anti-PUMA/BBC3 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA(145-159aa ADDLNAQYERRRQEE), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-02-16

Question

Does PA1313 anti-PUMA/BBC3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Z. Anderson

Verified customer

Asked: 2014-02-03

Answer

You can see on the product datasheet, PA1313 anti-PUMA/BBC3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-02-03

Size

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PA1313
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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