Rabbit IgG polyclonal antibody for Bcl-2-binding component 3(BBC3) detection. Tested with WB in Human;Mouse;Rat. Cited in 2 publication(s).
Anti-PUMA/BBC3 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
Intercellular Adhesion Molecule-2 (ICAM2) belongs to the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, containing 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. The ICAM2 protein has a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. ICAM2 mediates adhesive interactions vital for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and additional cellular interactions essential for immune response and surveillance.
|Product Name||Anti-PUMA/BBC3 Antibody
See all PUMA primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for PUMA/BBC3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. PUMA/BBC3 information: Molecular Weight: 20532 MW; Subcellular Localization: Mitochondrion . Localized to the mitochondria in order to induce cytochrome c release; Tissue Specificity: Ubiquitously expressed.|
|Cite This Product||Anti-PUMA/BBC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1313)|
|Similar Products From
|Anti-PUMA/BBC3 Antibody may replace the following items: sc 19187|sc 19188|sc 19189|sc 19190|sc 19191|sc 20534|sc 20536|sc 28226|sc 374223|sc 377015.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA(145-159aa ADDLNAQYERRRQEE), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For BBC3 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||27113|
|Species Of This Entry||Human|
|Protein Name||Bcl-2-binding component 3, isoforms 1/2|
|Alternative Names||PUMA|BCL2 binding component 3; bcl-2-binding component 3; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMAFLJ42994|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Acetylation; Methylation; Ubiquitination; Sumoylation; Deacetylation; Farnesylation; Glycosylation; Dephosphorylation|
|Subcellular Localization||Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release.|
|Gene Location||19q13.32, on Chromasome 19, gene sequence: NC_000019.10 (47220822..47232998, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For BBC3 (Source: Uniprot.org, NCBI)
|Protein Function||Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis (PubMed:11463391). Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis (PubMed:23340338).|
|Tissue Specificity||Ubiquitously expressed.|
|Interacting Proteins||BCL2; BCL2L2; MCL1; Mcl1|
|Research Areas||Apoptosis; Cancer; DNA Double Strand Break Repair; Signal Transduction; Tumor Suppressors|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Leukemia; Malignant Tumor Of Colon; Cell Transformation, Neoplastic; Malignant Neoplasm Of Breast; Colorectal Cancer; Mammary Neoplasms; Colorectal Neoplasms; Malignant Paraganglionic Neoplasm; Colonic Neoplasms; Neuroblastoma; Melanoma; Adenocarcinoma; Lymphoma; Lymphoid Leukemia; Lung Neoplasms; Genotoxic Stress|
|Related Pathways||Cell Death; Cell Cycle; Induction Of Apoptosis; Cell Cycle Arrest; Cell Growth; Rna Interference; Cell Proliferation; Mitochondrial Translocation; Pathogenesis; Sensitization; Methylation; Localization; Autophagy; Oncogenesis; Programmed Cell Death; Cell Differentiation; Dna Repair; Senescence; Apoptotic Process; Mitochondrial Outer Membrane Permeabilization|
|Background||The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.|
|Scientific References||PMID: 11463391 by Yu J., et al. PUMA induces the rapid apoptosis of colorectal cancer cells.|
PMID: 11463392 by Nakano K., et al. PUMA, a novel proapoptotic gene, is induced by p53.
Our Boster Quality Guarantee for Anti-PUMA/BBC3 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti PUMA(PA1313) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 21KD
Observed bind size: 21KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 2
|Zhao Y, Wu S, Wu J, Jia P, Gao S, Yan X, Wang Y. Cancer Biol Ther. 2011 Jan 1;11(1):95-107. Doi: 10.4161/Cbt.11.1.13960. Introduction Of Hypoxia-Targeting P53 Fusion Protein For The Selective Therapy Of Non-Small Cell Lung Cancer.||PubMed ID 21248471|
|Nerve growth factor reduces apoptotic cell death in rat facial motor neurons after facial nerve injury||PubMed ID 25630785|
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