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Product Info Summary
| SKU: | P01654 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody
SKU/Catalog Number
P01654
BM3960 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody catalog # P01654. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01654)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOH-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-LAT (Y191)
Reactive Species
P01654 is reactive to LAT in Human, Mouse, Rat
Observed Molecular Weight
38 kDa
Calculated molecular weight
27.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01654 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, rat thymus tissue, rat RH35 whole cell, mouse thymus tissue
Validation Images & Assay Conditions
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Western blot analysis of P-LAT using anti-P-LAT antibody (P01654).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-LAT antigen affinity purified monoclonal antibody (P01654) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-LAT at approximately 38 kDa. The expected band size for P-LAT is at 28 kDa.
Specific Publications For Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody (P01654)
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5 Customer Q&As for Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody
Question
Our lab were well pleased with the WB result of your anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody. However we have observed positive staining in platelet cell membrane using this antibody. Is that expected? Could you tell me where is LAT supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
From what I have seen in literature, platelet does express LAT. Generally LAT expresses in cell membrane. Regarding which tissues have LAT expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 15616553
Colon, Pubmed ID: 15489334
Leukemia, Pubmed ID: 9489702
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Thymus, Pubmed ID: 9529333
Boster Scientific Support
Answered: 2020-04-21
Question
We have been able to see staining in mouse leukocyte. Any tips? Is anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
According to literature leukocyte does express LAT. According to Uniprot.org, LAT is expressed in leukocyte, leukemia, thymus, bone marrow, colon, leukemic t-cell, platelet, liver, among other tissues. Regarding which tissues have LAT expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 15616553
Colon, Pubmed ID: 15489334
Leukemia, Pubmed ID: 9489702
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Thymus, Pubmed ID: 9529333
Boster Scientific Support
Answered: 2019-07-01
Question
I am interested in using your anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody for intracellular signal transduction studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Thanks for your inquiry. This P01654 anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-13
Question
We are currently using anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody P01654 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-10
Answer
The anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody (P01654) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-10
Question
We ordered your anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody for IF on liver a few months ago. I am using mouse, and We intend to use the antibody for ICC next. We need examining liver as well as leukemia in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
T. Wu
Verified customer
Asked: 2016-08-26
Answer
I looked at the website and datasheets of our anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody and I see that P01654 has been validated on mouse in both IF and ICC. Thus P01654 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-08-26
