Anti-Phospho-RIPK2 (S176) Antibody

Product Name

Anti-Phospho-RIPK2 (S176) Antibody

View all RIPK2 Antibodies

SKU/Catalog Number

P00818

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-Phospho-RIPK2 (S176) Antibody catalog # P00818. Tested in WB, IHC, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Cite This Product

Anti-Phospho-RIPK2 (S176) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00818)

Host

Rabbit

Contents

500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Immunogen

A synthesized peptide derived from human RIPK2 around the phosphorylation site of Ser176.

Reactive Species

P00818 is reactive to RIPK2 in Human, Mouse, Rat

Calculated molecular weight

61.2 kDa

Background of RIPK2

RIPK2(Receptor-interacting serine/threonine-protein kinase 2), also known as CARD3, CARDIAK, RICK, RIP2, is an?enzyme?that in humans is encoded by the?RIPK2?gene.?It has 540-amino acid protein in length.?Northern blot analysis revealed that RICK is expressed in various human tissues as 2.5- and 1.8-kb mRNAs that differ due to alternative polyadenylation. RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway. This gene encodes a member of the receptor-interacting protein(RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal?caspase recruitment domain(CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of?NF-kappa B?and inducer of apoptosis in response to various stimuli, CARDIAK(CARD-containing ICE-associated kinase) specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 subunit.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P00818 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information RIPK2

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