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Product Info Summary
| SKU: | P00097-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-Tau (S396) MAPT Rabbit Monoclonal Antibody
SKU/Catalog Number
P00097-2
BM4496 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Tau (S396) MAPT Rabbit Monoclonal Antibody catalog # P00097-2. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Tau (S396) MAPT Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00097-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FCO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Tau (S396)
Reactive Species
P00097-2 is reactive to MAPT in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
78.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00097-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
Positive Control
WB: human SH-SY5Y whole cell, mouse brain tissue
IHC: human breast carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Tau (S396) MAPT using anti-Phospho-Tau (S396) MAPT antibody (P00097-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin V antigen affinity purified monoclonal antibody (P00097-2) at 1:10000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Annexin V at approximately 54 kDa. The expected band size for Annexin V is at 54 kDa.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Tau (S396) Antibody.
Specific Publications For Anti-Phospho-Tau (S396) MAPT Rabbit Monoclonal Antibody (P00097-2)
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-Tau (S396) MAPT Rabbit Monoclonal Antibody
Question
We have observed staining in rat brain. Are there any suggestions? Is anti-Phospho-Tau (S396) Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
From what I have seen in literature brain does express MAPT. From what I have seen in Uniprot.org, MAPT is expressed in parietal lobe, brain, fetal brain, fetal brain cortex, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MAPT expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1512244, 2484340, 2495000, 2498079, 3131773, 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 2516729
Fetal brain cortex, Pubmed ID: 16443603
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-Phospho-Tau (S396) Rabbit Monoclonal antibody P00097-2 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
B. Moore
Verified customer
Asked: 2017-08-22
Answer
The anti-Phospho-Tau (S396) Rabbit Monoclonal antibody (P00097-2) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-22
Question
We have tried in the past anti-Phospho-Tau (S396) Rabbit Monoclonal antibody for WB on parietal lobe a few years ago. I am using human, and We want to use the antibody for IHC next. I would like examining parietal lobe as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
I viewed the website and datasheets of our anti-Phospho-Tau (S396) Rabbit Monoclonal antibody and it seems that P00097-2 has been tested on human in both WB and IHC. Thus P00097-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-22
Question
Our team were happy with the WB result of your anti-Phospho-Tau (S396) Rabbit Monoclonal antibody. However we have been able to see positive staining in leukemic t-cell cytoplasm using this antibody. Is that expected? Could you tell me where is MAPT supposed to be expressed?
T. Jones
Verified customer
Asked: 2015-09-04
Answer
From what I have seen in literature, leukemic t-cell does express MAPT. Generally MAPT expresses in cytoplasm, cytosol. Regarding which tissues have MAPT expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1512244, 2484340, 2495000, 2498079, 3131773, 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 2516729
Fetal brain cortex, Pubmed ID: 16443603
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2015-09-04
