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Product Info Summary
| SKU: | A00822Y52 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PKC Delta (phospho-Y52) PRKCD Antibody
SKU/Catalog Number
A00822Y52
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PKC Delta (phospho-Y52) PRKCD Antibody catalog # A00822Y52. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC Delta (phospho-Y52) PRKCD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00822Y52)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human PKC δ around the phosphorylation site of Tyrosine 52.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00822Y52 is reactive to PRKCD in Human, Mouse, Rat
Calculated molecular weight
77.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00822Y52 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of p-PKC δ (Y52) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with serum starvation(24h) Lane2:Raw264.7 cell lysate treated with serum starvation(24h) Lane3:H9C2 cell lysate treated with serum starvation(24h)
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Immunohistochemistry (IHC) analyzes of p-PKC δ (Y52) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Specific Publications For Anti-PKC Delta (phospho-Y52) PRKCD Antibody (A00822Y52)
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