Rabbit IgG polyclonal antibody for Cardiac phospholamban(PLN) detection. Tested with WB in Human;Mouse;Rat.
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-Phospholamban/PLN Antibody
See all PLN primary antibodies, ELISA kits and proteins
|Storage & Handling||For proper storage of Anti-Phospholamban/PLN Antibody: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for PHOSPHOLAMBAN/PLN detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. PHOSPHOLAMBAN/PLN information: Molecular Weight: 6109 MW; Subcellular Localization: Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane ; Single- pass membrane protein . Endoplasmic reticulum membrane; Single-pass membrane protein; Tissue Specificity: Heart muscle (at protein level).|
|Cite This Product||Anti-Phospholamban/PLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1309)|
|Specificity||Anti-Phospholamban/PLN Antibody (PA1309) reacts with Human, Mouse, Rat PLN, in native form and recombinant. Superfamily members of PLN are not reactive to PA1309.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human PLN (2-16aa EKVQYLTRSAIRRAS), identical to the related rat and mouse sequences.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-Phospholamban/PLN Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-Phospholamban/PLN Antibody (PA1309).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Anti-PLN antibody, PA1309, Western blotting
All lanes: Anti PLN (PA1309) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Predicted bind size: 18KD
Observed bind size: 18KD
Protein Target Info (Source: Uniprot.org)
|Protein Name||Cardiac phospholamban|
|Tissue Specificity||Heart muscle (at protein level). .|
|Alternative Names||Cardiac phospholamban;PLB;PLN;PLB;|
|Subcellular Localization||Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane ; Single- pass membrane protein . Endoplasmic reticulum membrane; Single-pass membrane protein.|
|Molecular Weight||6109 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. .|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2+ pump in cardiac muscle and skeletal muscle cells. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Phospholamban is also expressed in slow-twitch skeletal muscle and some smooth muscle cells. McTiernan et al.(1999) observed that human ventricle and quadriceps displayed high levels of phospholamban transcripts and proteins, with markedly lower expression observed in smooth muscles, while the right atrium also expressed low levels of phospholamban. The structure of the human phospholamban gene closely resembles that reported for chicken, rabbit, rat, and mouse. Comparison of the human to other mammalian phospholamban genes indicated a marked conservation of sequence for at least 217 bp upstream of the transcription start site.|
Other Recommended Resources
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.