Anti-Phospholamban/PLN Antibody

Phospholamban antibody

Boster Bio Anti-Phospholamban/PLN Antibody catalog # PA1309. Tested in WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: PA1309
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Phospholamban/PLN Antibody

View all Phospholamban Antibodies

SKU/Catalog Number

PA1309

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Phospholamban/PLN Antibody catalog # PA1309. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospholamban/PLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1309)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PLN, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1309 is reactive to PLN in Mouse, Rat

Applications

PA1309 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

24 kDa, 12 kDa

Calculated molecular weight

6.109kDa

Background of Phospholamban

Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2+ pump in cardiac muscle and skeletal muscle cells. The subsequent activation of the Ca (2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Phospholamban is also expressed in slow-twitch skeletal muscle and some smooth muscle cells. McTiernan et al. (1999) observed that human ventricle and quadriceps displayed high levels of phospholamban transcripts and proteins, with markedly lower expression observed in smooth muscles, while the right atrium also expressed low levels of phospholamban. The structure of the human phospholamban gene closely resembles that reported for chicken, rabbit, rat, and mouse. Comparison of the human to other mammalian phospholamban genes indicated a marked conservation of sequence for at least 217 bp upstream of the transcription start site.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For PLN (Source: Uniprot.org, NCBI)

Gene Name

PLN

Full Name

Cardiac phospholamban

Weight

6.109kDa

Superfamily

phospholamban family

Alternative Names

CMD1PPLBcardiac phospholamban; phospholamban PLN CMD1P, CMH18, PLB phospholamban cardiac phospholamban

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PLN, check out the PLN Infographic

PLN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PLN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Phospholamban/PLN Antibody

Question

I am looking for to test anti-Phospholamban/PLN antibody PA1309 on human liver for research purposes, then I may be interested in using anti-Phospholamban/PLN antibody PA1309 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

The products we sell, including anti-Phospholamban/PLN antibody PA1309, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-06

Question

Will PA1309 anti-Phospholamban/PLN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

You can see on the product datasheet, PA1309 anti-Phospholamban/PLN antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-28

Question

Would anti-Phospholamban/PLN antibody PA1309 work on primate WB with liver?

Verified Customer

Verified customer

Asked: 2019-03-11

Answer

Our lab technicians have not validated anti-Phospholamban/PLN antibody PA1309 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Phospholamban/PLN antibody PA1309 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-11

Question

Will anti-Phospholamban/PLN antibody PA1309 work for WB with liver?

Verified Customer

Verified customer

Asked: 2018-05-09

Answer

According to the expression profile of liver, PLN is highly expressed in liver. So, it is likely that anti-Phospholamban/PLN antibody PA1309 will work for WB with liver.

Boster Scientific Support

Answered: 2018-05-09

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Phospholamban/PLN antibody PA1309. Let me know if you need anything else.

A. Singh

Verified customer

Asked: 2016-03-29

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-03-29

Order DetailsPrice
PA1309

100μg

$370
PA1309-10ug

10μg sample (liquid)

$99
PA1309-Biotin

100 μg Biotin conjugated

$570
PA1309-Cy3

100 μg Cy3 conjugated

$570
PA1309-Dylight488

100 μg Dylight488 conjugated

$570
PA1309-Dylight550

100 μg Dylight550 conjugated

$570
PA1309-Dylight594

100 μg Dylight594 conjugated

$570
PA1309-FITC

100 μg FITC conjugated

$570
PA1309-HRP

100 μg HRP conjugated

$570
PA1309-APC

100 μg APC conjugated

$670
PA1309-PE

100 μg PE conjugated

$670
PA1309-carrier-free

Carrier Free

$370

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