Mouse Rantes ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Rantes. 96wells/kit, with removable strips. Cited in 3 publication(s).
Mouse Rantes ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates. serum and plasma(heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
|Product Name||Mouse Rantes ELISA Kit PicoKine™
See all Ccl5 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse Rantes/CCL5 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates. serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Rantes/CCL5 tissue specificity: T-cell and macrophage specific.|
|Cite This Product||Mouse Rantes ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0495)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates. serum and plasma(heparin, EDTA, citrate).|
|Immunogen||Expression system for standard: E.coli; Immunogen sequence: S24-S91|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
|Protein Name||C-C motif chemokine 5|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells. .|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Mouse Rantes ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0495-CAP||96-well plate precoated with anti-Mouse Ccl5 antibody||1|
|EK0495-ST||lyophilized recombinant Mouse Ccl5 standard||10ng/tube|
|EK0495-DA||biotinylated anti-Mouse Ccl5 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0495 Mouse Rantes ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse Rantes ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse Rantes ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 3
|Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...||PubMed ID 26022197|
|In vitro modeling of COPD inflammation and limitation of p38 inhibitor - SB203580||PubMed ID 27199554|
|Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway||PubMed ID 27576490|
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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