Click image to see more details
Product Info Summary
| SKU: | A04601-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PLOD2 Antibody
SKU/Catalog Number
A04601-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-PLOD2 Antibody catalog # A04601-2. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-PLOD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04601-2)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human PLOD2 recombinant protein (Position: V455-N541).
Reactive Species
A04601-2 is reactive to PLOD2 in Human, Rat
Calculated molecular weight
84.7 kDa
Background of PLOD2
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04601-2 is guaranteed for ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400
Immunoprecipitation, 1:50
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 1:100-1000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PLOD2 using anti-PLOD2 antibody (A04601-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLOD2 antigen affinity purified polyclonal antibody (A04601-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLOD2 at approximately 85 kDa. The expected band size for PLOD2 is at 85 kDa.
Click image to see more details
IHC analysis of PLOD2 using anti-PLOD2 antibody (A04601-2).
PLOD2 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-PLOD2 Antibody (A04601-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-PLOD2 Antibody (A04601-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PLOD2 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-PLOD2 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
