Product Info Summary
SKU: | PA1952 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytochrome P450 Reductase/POR Antibody
View all POR/Cytochrome P450 Reductase Antibodies
SKU/Catalog Number
PA1952
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytochrome P450 Reductase/POR Antibody catalog # PA1952. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 Reductase/POR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1952)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human POR, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1952 is reactive to POR in Human, Mouse, Rat
Applications
PA1952 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
76.69kDa
Background of POR/Cytochrome P450 Reductase
POR is a membrane-boundenzyme required for electron transfer from NADPH to cytochrome P450 in the endoplasmic reticulum of theeukaryotic cell. The gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry, 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-POR antibody, PA1952, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Spleen Tissue Lysate
Lane 5: A549 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SKOV Cell Lysate
Lane 8: MCF-7 Cell Lysate
Click image to see more details
Anti-POR antibody, PA1952, IHC(P)
IHC(P): Human Placenta Tissue
Click image to see more details
Anti-POR antibody, PA1952, ICC
ICC: HELA Cell
Click image to see more details
Figure 4. IHC analysis of POR using anti-POR antibody (PA1952).
POR was detected in paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POR Antibody (PA1952) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For POR (Source: Uniprot.org, NCBI)
Gene Name
POR
Full Name
NADPH--cytochrome P450 reductase
Weight
76.69kDa
Superfamily
NADPH--cytochrome P450 reductase family
Alternative Names
CPR; CYPOR; CYPORFLJ26468; Cytochrome P450 Reductase; DKFZp686G04235; EC 1.6.2.4; NADPH--cytochrome P450 reductase; NADPH-dependent cytochrome P450 reductase; P450 (cytochrome) oxidoreductase; P450R; POR POR CPR, CYPOR, P450R cytochrome p450 oxidoreductase NADPH--cytochrome P450 reductase|NADPH--hemoprotein reductase|NADPH-dependent cytochrome P450 reductase|P450 (cytochrome) oxidoreductase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POR, check out the POR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 Reductase/POR Antibody (PA1952)
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