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Product Info Summary
| SKU: | A11425 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IFN- epsilon Antibody
SKU/Catalog Number
A11425
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IFN- epsilon Antibody catalog # A11425. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IFN- epsilon Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11425)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human IFNE. AA range:159-208
Reactive Species
A11425 is reactive to IFNE in Human, Mouse
Calculated molecular weight
24.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11425 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western blot analysis of Jurkat cells using IFN-Epsilon Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from Jurkat cells, using IFNE Antibody.
Specific Publications For Anti-IFN- epsilon Antibody (A11425)
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Customer Q&As
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5 Customer Q&As for Anti-IFN- epsilon Antibody
Question
We are currently using anti-IFN- epsilon antibody A11425 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
The anti-IFN- epsilon antibody (A11425) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-19
Question
Will anti-IFN- epsilon antibody A11425 work on dog WB with ectocervix?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
Our lab technicians have not validated anti-IFN- epsilon antibody A11425 on dog. You can run a BLAST between dog and the immunogen sequence of anti-IFN- epsilon antibody A11425 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog ectocervix in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ectocervix using anti-IFN- epsilon antibody A11425. Let me know if you need anything else.
L. Carter
Verified customer
Asked: 2019-03-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-14
Question
My question regarding product A11425, anti-IFN- epsilon antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Jones
Verified customer
Asked: 2015-10-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11425 anti-IFN- epsilon antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-10-28
Question
you antibody to test anti-IFN- epsilon antibody A11425 on human ectocervix for research purposes, then I may be interested in using anti-IFN- epsilon antibody A11425 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Taylor
Verified customer
Asked: 2013-07-18
Answer
The products we sell, including anti-IFN- epsilon antibody A11425, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-07-18
