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- Table of Contents
Cyp17a1 antibody (A00615-3) performs well in ELISA, IF, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IF analysis of Cyp17a1 using anti-Cyp17a1 antibody (A00615-3).
Cyp17a1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Cyp17a1 Antibody (A00615-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Cyp17a1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Cyp17a1 Antibody (A00615-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband®
Cyp17a1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IF, IHC, WB |
Tested Applications
Cyp17a1 antibody was positively detected by WB in:
rat testis tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Cyp17a1 antibody was positively detected by IHC in:
mouse testis tissue, rat testis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Cyp17a1 antibody was positively detected by IF in:
mouse testis tissue, rat testis tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunofluorescence
2μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Tested
Recommend
Mouse
Tested
Tested
Tested
Recommend
Product Information
Product Name
Anti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband®
View all CYP17A1 Antibodies
View all CYP17A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Cyp17a1 recombinant protein (Position: Q80-R363).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
57 kDa
Calculated Molecular Weight
57.6 kDa
Background of Cyp17a1
Cytochrome P450 17A1, also called steroid 17α-monooxygenase, is an enzyme of the hydroxylase type that in humans is encoded by the CYP17A1 gene on chromosome 10. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.
Cite This Product
Anti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00615-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
79%
Moderate Homology
Human
60%
Low homology
Rabbit
60%
Low homology
Monkey
59%
Low homology
Dog
59%
Low homology
Sheep
58%
Low homology
Cow
57%
Low homology
Zebrafish
46%
Low homology
Drosophila
29%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Cyp17a1 antibody
Immunohistochemistry (IHC) protocol for Cyp17a1 antibody
Immunofluorescence (IF) protocol for Cyp17a1 antibody
Publications
Application:
Species:
Neuregulin 4 improves white adipose tissue inflammation in mice with polycystic ovary syndrome
Published: 2026-06-10
Journal: PEPTIDES
Application: IF
Impact Factor: 3.50
Species: Mouse
PMID: 42269722
Semaglutide Alleviates Ovarian Oxidative Stress and Autophagy via the PI3K/AKT/mTOR Pathway in Mice with Polycystic Ovary Syndrome
Published: 2025-05-22
Journal: Drug Design Development and Therapy
Application: IF
Impact Factor: 5.10
Species: Mouse
CYP17 gene plays a key role in goose genital growth by influencing the testosterone level at puberty
Published: 2019-12-11
Journal: POULTRY SCIENCE
Application: IHC
Impact Factor: 2.03
Species: Goose
PMID: 29462369
Effects of six priority controlled phthalate esters with long-term low-dose integrated exposure on male reproductive toxicity in rats
Published: 2017-01-12
Journal: FOOD AND CHEMICAL TOXICOLOGY
Application: WB
Impact Factor: 3.78
Species: Rat
PMID: 28089693
Endogenous release of female hormones from co-microencapsulated rat granulosa and theca cells
Published: 2013-11-10
Journal: BIOMEDICAL MICRODEVICES
Application: IHC
Impact Factor: 2.72
Species: Rat
PMID: 24212501
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Product Promise
We promise Anti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00615-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026